Investigating the Efficacy of Replacement Therapy for Masseter Muscle Sarcopenia Using Metabolome Analysis

使用代谢组分析研究替代疗法治疗咬肌肌少症的疗效

• 批准号: 22K17145
• 负责人: 加藤 禎彬
• 金额: $ 1.75万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K17145/
• 关键词: サルコペニア; オーラルフレイル; 咬筋の加齢変化; 筋肉の加齢変化; メタボローム解析; 咬筋の老化変化; 代謝物質

老年歯科医学分野では口腔機能低下症の診断基準が制定され予防的な考えが注目されている。私たちのグループはこれまでに老化促進モデルマウス(SAM)の咬筋は老化で組織学的な形態的変化を認めることを明らかにした。今回われわれはSAMの咬筋に対してメタボローム解析を行った。メタボローム解析は12週齢、40週齢、55週齢のSAMの咬筋各5匹ずつに対して行った。咬筋の主成分分析（以下、PCA）では第一主成分で各週齢を効果的にかつ明確に分離でき骨格筋の老化が筋肉の全体的な代謝産物プロファイルに有意な変化を認めた。階層的クラスター解析（HCA）では40週齢から55週齢にかけて明らかな代謝変動を認めた。40週齢と55週齢で変動を認めた代謝物絶対定量値で有意差を認めたのは36物質で上昇した代謝物は20物質、減少した代謝物は16物質であった。さらに因子負荷量で第一主成分に影響を及ぼすものは上昇10物質、減少9物質であった。中でもPolyamine代謝経路、Purine代謝経路で有意な変動を認めた。Polyamine代謝経路は細胞増殖や、たんぱく質合成、核酸合成に関与している代謝経路でありS-AdenosylmethionineとSpermidineの有意な上昇を認めた。Purine代謝経路はプリンヌクレオチドの代謝やATP再合成に関与する代謝経路でありIMP、GMP、GDP、Hypoxanthineの有意な上昇とGTP、ATPの有意な減少を認めた。神経伝達物質のCholineの上昇を認めた。ATP合成に使われるPhosphocreatineと反応後生成物のCreatinineの減少を認めた。解糖系に関与する3-Phosphoglyceric acid、2-Phosphoglyceric acid、phosphoenolpyruvic acidの減少を認めた

Geriatric dentistry is a field of medicine that focuses on the development of diagnostic criteria for oral hypofunction. The changes of histological morphology of aging tissue were identified. This time around, the SAM's bite was analyzed. The analysis of SAM in 12 weeks, 40 weeks and 55 weeks was carried out in 5 different ways. Principal component analysis (PCA) of the tendon (hereinafter referred to as "the principal component analysis") identifies the first principal component, the first principal component, the second principal component, the third principal component, the third principal component, the fourth principal component, the third principal component, the fourth principal component, the principal component, the fourth principal component, the principal component, the fourth principal component, the principal component, the third principal component, the principal component, the principal Hierarchical analysis (HCA) is a 40-week cycle and a 55-week cycle. 40 weeks ago, 55 weeks ago, the metabolites were identified and quantified. 36 substances were identified. 20 substances were identified and 16 substances were identified. The first principal component of the load is increased by 10 substances and decreased by 9 substances. In the middle, the Polyamine metabolic pathway and Purine metabolic pathway are intentionally activated. Polyamine metabolic pathway is related to cell proliferation, plasma synthesis, nucleic acid synthesis and the intentional rise of S-adenosylmethionine and Spermidine. Purine metabolic pathway is related to ATP biosynthesis and IMP, GMP, GDP, Hypoxanthine intentional increase and ATP intentional decrease. The rise of Choline in the brain is recognized. ATP synthesis is the result of the reduction of phosphocholine and Creatinine. Sugar hydrolysis system is related to the reduction of 3-phosphoglycolic acid, 2-phosphoglycolic acid and phosphoenolbutyric acid.