次世代シークエンサーを用いた簡易的検体採取による口腔癌新規スクリーニング法の確立

使用下一代测序仪进行简单标本采集，建立新的口腔癌筛查方法

• 批准号: 22K17150
• 负责人: 纐纈 衆
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K17150/
• 关键词: 口腔癌; 口腔細菌叢; メタゲノム解析

本研究では、次世代シークエンサーを用い、細菌叢のメタゲノム解析による広範囲顎骨支持型装置の治療効果判定・口腔機能回復の予後予測解析を目指す。次世代シークエンサーの登場ならびにバイオインフォマティクスによる解析技術の向上により、消化器疾患のさらなる治療展開が期待されている。口腔は唾液などの検体が極めて採取しやすい器官である。メタゲノム解析での顎骨再建治療術後の補綴治療効果の判定が達成されれば、より効果的・非侵襲・効果的・簡易的な診断・治療が期待できる。以下の3つの方法により、細菌叢分析を臨床応用に発展させる。(1) 口腔癌患者再建術患者の口腔検体による細菌叢メタゲノム解析による評価、　(2) 広範囲顎骨支持型装置による口腔機能改善に伴う補綴治療前後での細菌叢変化の評価、　(3) インプラント治療合併症やオーラルフレイルに関連する菌腫に対し、新たな治療戦略(予防・診断・治療)を確立現在、口腔検体148検体の唾液・歯垢・舌苔を採取し，それぞれの細菌叢を次世代シークエンサーによる16SrRNA解析を行い両群の比較を行った。ある特定の菌種、特に網レベルでの解析、種レベルでの解析結果で、腫瘍進展・発症と相関があることが明らかになった。また口腔検体の間、唾液・歯垢・舌苔の間でも解析された細菌叢に違いがあり、今後は腫瘍発癌により特異的な菌種の同定、腫瘍予後や病期、組織学的検索結果との相関を検索する予定である。

The purpose of this study is to determine that the dental machine can be used to determine that the dental machine can be used to analyze the results of the treatment of bone-supported devices in the next generation. In the next generation, we are looking forward to the opening of the treatment of digestive diseases and the development of technology. Oral saliva, saliva, body, body, saliva, saliva, saliva, After the treatment of bone remodeling, the results were determined to be easy to treat and look forward to non-invasive treatment. The following "3" methods are used for the analysis of bacteria and bacteria. The main results are as follows: (1) Oral cancer patients were treated with reconstructive surgery. (1) Oral cancer patients were treated with oral reconstruction. the results were as follows: (1) Oral cancer patients were treated with reconstructive surgery. (1) Oral cancer patients were treated with oral cancer. (1) Oral cancer patients were treated with reconstructive surgery. (2) Oral cavity machine with bone support device can improve the treatment of bacteria before and after treatment. The new treatment strategy (for prevention and treatment) is to make sure that it is present, and the saliva and tongue coating are removed from 148 body parts of the oral cavity. The next generation of bacteria is called "16SrRNA parsing", "group" and "row". Analyze the results of specific bacteria, special network analysis, analysis of the results of the test, and identify the results of the disease. The oral and salivary smear and tongue coating tests were used to analyze the bacteria in the oral cavity, saliva and tongue coating, the identification of the bacterial species, the later stage of the disease, and the histology of the cancer patients in the future. results the results of correlation analysis showed that the results of correlation analysis showed that the clinical characteristics of cancer patients were the same.