The interplay of EBV-induced γδ and αβ T cells during chronic EBV reactivation, PTLD, and EBV-directed cellular therapy in transplant recipients (B11*)

EB病毒(Epstein Barr Virus，EBV)是移植后慢性感染和肿瘤的病因。在适应性抗EBV T细胞反应方面已经获得了巨大的洞察力，但最近的研究结果表明，先天性T细胞免疫在移植后的病毒感染中起着关键作用。本项目旨在通过功能鉴定和下一代测序技术在单细胞水平上表征免疫EB病毒诱导的T细胞网络，以了解EB病毒在免疫活性和免疫抑制患者中诱导的先天(γδ)和适应性(αβ)免疫的相互作用。最后，分析参数将被翻译到接受EBV特异性T细胞转移的患者，旨在改善采用的抗EBV T细胞治疗理念。

Epstein Barr Virus (EBV) is the cause for chronic infection and tumors after transplantation. While enormous insights have been gained into adaptive anti-EBV T cell responses recent results suggest a pivotal role of innate T cell immunity in viral infections after transplantation. This project aims at understanding the interplay of innate (γδ) and adaptive (αβ) EBV-induced T cell immunity during EBV infection in immunocompetent and immunosuppressed patients through functional characterization and next generation sequencing technologies to characterize the immune EBV-induced T cell network on single cell level. Finally, analyses parameters will be translated to patients receiving EBV-specific T cell transfer aiming at improving adoptive anti-EBV T cell therapy concepts.