Calpain 15 activity in immune regulation

Calpain 15 的免疫调节活性

• 批准号: 406318528
• 负责人: Professor Dr. Andreas Pichlmair
• 金额: --
• 依托单位: Institut für Virologie (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/406318528?language=en
• 关键词: Calpain; 15; activity; immune; regulation

The innate immune system consists of a network of sensors and signalling molecules. The regulation of this system is very complex and only partially understood. However, all circulating viruses evolutionary evolved to perturb this system in order to propagate their spread. We used an affinity proteomics approach to identify hitherto unknown components of the innate immune system. Through these screens we identified the protease calpain 15 (CAPN15) as interaction partner of the immunomodulatory protein ML of Thogotovirus. Preliminary experiments show that CAPN15 interacts with TRAF2 and DIABLO, which are components of the TNF-dependent NF-kB signaling pathway. Functionally CAPN15 regulates TNF-dependent signaling, but does not influence signaling elicited by IL1-beta. Here we propose to study the function of CAPN15 for the innate immune system. We plan to use a combination of functional and proteomic analyses and to study the function of CAPN15 in vivo.

先天免疫系统由传感器和信号分子网络组成。这一制度的规则非常复杂，而且只被部分理解。然而，所有的循环病毒都进化为扰乱这个系统，以传播它们的传播。我们使用亲和蛋白质组学方法来鉴定迄今未知的先天免疫系统成分。通过这些筛选，我们发现蛋白酶calpain 15 （CAPN15）是Thogotovirus免疫调节蛋白ML的相互作用伙伴。初步实验表明，CAPN15与TRAF2和DIABLO相互作用，而TRAF2和DIABLO是tnf依赖性NF-kB信号通路的组成部分。功能上，CAPN15调节tnf依赖性信号，但不影响由il - 1- β引发的信号。本文拟研究CAPN15在先天免疫系统中的功能。我们计划结合功能和蛋白质组学分析来研究CAPN15在体内的功能。