Transcriptional complexes and chromatin reorganization in lineage choices and differentiation of embryonic stem cells (A16*)

胚胎干细胞可以分化为几乎所有类型的体细胞。细胞类型特异性基因表达模式的建立需要多种因素的综合作用，包括信号分子、转录因子和表观遗传修饰因子。在不同的提示下，转录因子结合细胞类型特异的增强子，并与辅因子和表观遗传修饰物一起在表观遗传和转录编程中发挥关键作用，指导胚胎干细胞的谱系选择和分化。这项拟议的研究计划的目的是描述控制胚胎干细胞心血管和造血谱系选择和心血管分化的转录和表观遗传学事件。

Embryonic stem (ES) cells can differentiate into virtually all somatic cell types. The establishment of cell type-specific gene expression patterns requires integrated activity of multiple factors including signaling molecules, transcription factors and epigenetic modifiers. Upon different cues, transcription factors bind cell type-specific enhancers and together with cofactors and epigenetic modifiers play key roles in epigenetic and transcriptional programming instructing the lineage choices and differentiation of embryonic stem cells. The objective of the proposed research program is to characterize the transcriptional and epigenetic events controlling cardiovascular versus hematopoietic lineage choice and cardiovascular differentiation of embryonic stem cells.