Development of functional evaluation for cartilagenous matrix using the measurement of ^1H-NMR and viscoelasticity

为了测量软骨组织的~1H-核磁共振弛豫时间和粘弹模数，在强磁场下安装了粘弹谱仪(型号：JNM 60E FSE，JEOL，日本东京)。本文分析了牛膝关节股骨髁全层软骨和牛椎间盘髓核的~1H-核磁共振松弛时间与粘弹模数的关系。为了适应核磁共振磁体的狭窄空间，设计了粘弹性光谱仪的测量探头，将机械换能器系统由水平地层改为垂直地层。动态力发生器由在线计算机系统(型号：PC9801VM2型，日本东京，东京)控制，并对通过软骨的检测信号进行分析，由计算机计算粘弹模数。对于关节软骨，水的1H-…松弛时间为粘弹模数主要随基质中水分含量的变化而变化。在应力疲劳试验过程中，随着基质中的水外溢到基质外空间，1H-核磁共振弛豫时间增加，粘弹性模量值也增加到平台值。由于样品中相对增加的自由水含量比其基质中的结合水具有更长的1H-核磁共振弛豫时间，在此过程中观察到的弛豫时间最终增加。髓核中的粘弹性依赖于水的迁移率，而水的迁移率由1H-核磁共振弛豫时间表示。制备了含有两种不同量糖胺多聚糖的奶牛DISO髓核。正常组的1H-核磁共振松弛时间与粘弹模数呈线性关系，而酶变性组则失去了线性关系，粘弹模数似乎不仅依赖于水的迁移率，而且还依赖于胶原纤维的弹性。提示可以通过测量基质中水的迁移率来估计软骨组织的基质排列紊乱和功能评价。较少

For the measurement of 1H-NMr relaxation times and visocelastic modules of cartilagenous tissue, viscoelastic spectrometer was arrenged and set up in the strong magnetic field of NMR devices( Model: JNM 60E FSE, JEOL, Tokyo, Japan). Relationship of 1H-NMR relaxation times and viscoelastic modules were analized in the full depth cartilage prepared from the femoral condyles of the knee joints of the cow, and in the nucleus pulposus of the intervertebral discs of the cow. The measuring probe of the viscoelastic spectrometer was designed to fit the narrow space in the NMR magnet, so that the mechanical transducer systems was changed into vertical formation from horizontal formation. Dynamic force generators were controlled by on-line computer system( Model: PC9801VM2, NEC, Tokyo, Japan) and the recieved signals of the detected fofce through the cartilage were analized and the viscoelastic modules were calculated by the computer.As for the joint cartilage, 1H-NMR relaxation times of water a … More nd viscoelastic modules were changed mainly with the change in the water contents in the matrix. As the water in the matrix exdated to the extra-matrix space during the stress fatigue test, 1H-NMR relaxation times increased and viscoelastic modules also increased to the plateau values. Because the free water fraction relativelly increased in the sampling area had longer 1H-NMR relaxation times than the bound water in its matrix, the observed relaxation times increased in this process finally. Viscoelastic property in the nucleus pulposus depended on the mobilities of water, which were represented by 1H-NMR relaxation times. Nucleus pulposus of the cow disos containing two different amount of glycosaminoglycans were prepared. The results of normal group indicated the liner relationship between 1H-NMR relaxation times and viscoelastic modules, while in the enzimatically degenerated group the liner relationship was lost and viscoelastic modules seemed to depended not only on the mobilities of water but also on the elasticity of collagen fiber.In conclusion, glycosaminoglycans plays an important part in the viscoelastic property of the cartilage by retaining water and resrticting its mobilities. It was suggested that the estimation of matrix derangement and functional evaluation of cartilagenous tissues could be possible by measuring of the mobilities of water in its matrix. Less