イネ初期胚発生で発現するホメオボックス遺伝子HOS13の機能解析

早期水稻胚胎发育过程中表达的同源盒基因HOS13的功能分析

• 批准号: 10760192
• 负责人: 伊藤 幸博
• 金额: $ 1.28万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10760192/
• 关键词: イネ; 胚発生; ホメオボックス遺伝子

植物は、受精卵からだけでなく分化した細胞からも脱分化を経て完全な個体を形成するが、その遺伝的制御機構は分子レベルではほとんど明らかにされていない。本研究の目的は、球状胚で強く発現し器官分化が見られる直前から発現が見られなくなったイネのKN1型ホメオボックス遺伝子HOS13の胚発生における機能を明らかにすることである。まず、カリフラワーモザイクウィルス35Sプロモーターを用いてHOS13をセンス方向およびアンチセンス方向に発現するコンストラクトをイネに導入した。しかし、いずれも何ら異常は見られなかった。それに対し、シュートメリステムで特異的に発現するKNI型ホメオボックス遺伝子OSH1、OSH15、OSH71を過剰発現させた場合、正常な再分化が阻害され、未分化なまま増殖を続けた。また、アンチセンスを発現するコンストラクトを導入した場合には、葉に形態異常が見られた。これらのKN1型ホメオボックス遺伝子がコードするホメオドメインは非常によく保存されているが、HOS13では動物も含めて全てのホメオドメインに例外なく保存されているアミノ酸が他のアミノ酸に置換されている。このアミノ酸はDNAへの結合に重要であることが知られている。従って、H0S13はこの1アミノ酸の置換を引き起こす変異のため機能を失った遺伝子であるか、あるいは他のKN1型ホメオボックス遺伝子とは大きく異なった機能を持つ遺伝子に変化したと推定された。

The plant and fertilized eggs are not differentiated, the cells are dedifferentiated, the complete body is formed, and the molecules of the control mechanism of the zygote are known to show that the disease is not normal. The purpose of this study is to detect the differentiation of organs in globular embryos. In this study, globular embryos were used to detect the differentiation of organs. In this study, globular embryos were used to detect the differentiation of organs. In this study, globular embryos were used to detect the differentiation of organs. In this study, globular embryos were used to detect the differentiation of organs. In this study, globular embryos were used to detect the differentiation of organs. In this study, globular embryos were used to detect the differentiation of organs. In this study, globular embryos were used to detect the differentiation of organs in order to detect the differentiation of organs. You can see that you are not in the same direction as you are. You can see that you are in the direction of the HOS13. I don't know what to say. I don't know. I don't know. OSH1, OSH15 and OSH71 were detected in KNI type antigens, normal redifferentiation and undifferentiated phenotypes, respectively. In the first place, please tell me that you are not aware of the situation, and that you are in a bad situation. In the case of the KN1 model, there is an exception in the case of an exception, which is not a good thing to save a lot of food. In the case of an exception, there is an exception in the case of an animal that contains a whole set of materials, such as the one in which you want to save, and the HOS13 in which you can save everything. This is the most important thing to know about the importance of the DNA. This is the first time that the health care system has been found to be in good condition. In this case, it is necessary to determine whether or not the KN1 type of machine is responsible for the health of the machine. In this case, it is important to know that the KN1 type of machine has the ability to modify the presumption of the disease.

项目成果

N. Sentoku, Y. Sato, N. Kurata, Y. Ito, H. Kitano, M. Matsuoka: "Regional expression of the rice KN1-type homeobox gene family during embiyo, shoot and flower development"Planet Cell. 11. 1651-1663 (1999)

N. Sentoku、Y. Sato、N. Kurata、Y. Ito、H. Kitano、M. Matsuoka：“水稻 KN1 型同源框基因家族在胚胎、芽和花发育过程中的区域表达”Planet Cell。

Y. ITO, M. Eiguchi, N. Kurata: "Expression of novel homeobox genes in early embryogenesis in rice"Biochimica et Biophysica Acta. 1444. 445-450 (1999)

Y. ITO、M. Eiguchi、N. Kurata：“水稻早期胚胎发生中新型同源盒基因的表达”Biochimica et Biophysicala Acta。