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ミトコンドリアDNAを用いたアジア産クワガタムシ科各属の系統解析

使用线粒体 DNA 对亚洲石竹科各属进行系统发育分析

基本信息

批准号：
09740639
负责人：
荒谷邦雄
金额：
$ 1.15万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

本研究では、著しい性的二型の存在などのために、形態形質に基づく系統解析を行うには大きな困難が伴うクワガタムシ科に関して、DNAに基づく系統解析を試みた。まず、最も分類学的な取り扱いが混乱しているクワガタ属内の系統関係について、日本および台湾産の種を材料にRAPD法を用いて解析したところ、アカアシクワガタ亜属の単系統性が支持された一方で、コクワガタ亜属が側系統である可能性が示唆された。次に、クワガタ属を含むDorcini族に関して、ミトコンドリアDNAのCOI遺伝子の439塩基対の配列に基づく系統解析を行なった。その結果、本族の中ではまずオニクワガタ属が、続いてノコギリクワガタ属が分岐したことが示唆され、クワガタ属の単系統性も支持された。また、クワガタ属内の系統関係は、概ね先のRAPD法の結果と一致した。しかし、COI遺伝子は、進化速度が速く、第3コドンのトランジションの変異が飽和している可能性が高いなどの問題があることがわかった。これらの結果を踏まえ、最後に、進化速度が比較的遅いとされるミトコンドリアl6SリボソームRNAの約480塩基対の配列を用いて、上述の属にツヤハダクワガタ属、ルリクワガタ属、マグソクワガタ属などを加え、クワガタムシ科全体の系統解析を行なった。その結果、クワガタムシ科の中ではツヤハダクワガタ属が最も早く分岐し、ついでマグソクワガタ属やルリクワガタ属が分岐したことが示唆された。一方、性的二型の著しいLucanini族とDorcini族が単系統をなすことも判明した。Dorcini族内の系統関係は概ねRAPD法やCOI遺伝子に基づく解析の結果と一致したが、オニクワガタ属がDorcini族からはずれ、Lucanini族のミヤマクワガタ属と単系統群を形成することが極めて高い確率で示唆された。これらの解析結果は、クワガタムシ科の系統分類学のみならず、本科における性的二型や配偶・繁殖行動、食性進化などを論ずる上でも極めて興味深い話題を提供するものである。

In this study, the existence of two types of reverse, middle and middle nature, morphological and qualitative analysis of basic system, and the analysis of DNA basic system were studied. The phylogenetic relationships within the genus were analyzed by RAPD, and the possibility of phylogenetic relationships within the genus was demonstrated by RAPD. The 439 base pairs of DNA and COI genes were analyzed for the first time. The results of this study are as follows: (1) The family is divided into two groups: (2) The family is divided into two groups: (3) The family is divided into two groups: (4) The family is divided into three groups: (5) The family is divided into three groups: (6) The family is divided into three groups: (7) The family is divided into three groups: (8) The family is divided into three groups: (9) The family is divided into three groups: (1) The family is divided into three groups: (9) The family is divided into three groups: (1) The family is divided into three groups: (9) The family is divided into three groups: (1) The family is divided into three groups: (9) The family is divided into three groups The results of RAPD method were consistent with those of the previous methods. The evolution speed of COI gene is high, the variation of the third node is high, and the possibility of the third node is high. The results of this study were as follows: 1. The evolution speed was compared with that of other species. 2. The alignment of about 480 base pairs of RNA was used. 3. The system analysis of all species was carried out. The result of this is that we have to be careful about what we do. The two types of one type and the other type of one type are Lucanini family and Dorcini family. The phylogenetic relationships of Dorcini family were analyzed by RAPD and COI gene analysis. The results were consistent with those of Dorcini family and Lucanini family. The results of this analysis are as follows: (1) The taxonomic classification of the family,(2) the sexual dimorphism,(3) the reproductive behavior,(4) the evolution of the diet,(5) the topic of deep interest,(6) the topic of deep interest,(7) the topic of deep interest,(8) the topic of deep interest,(9) the topic of deep interest,(10) the topic of deep interest,(11) the topic of deep interest,(12) the topic of deep interest,(13) the topic of deep interest,(14) the topic of deep interest,(15) the topic of deep interest,(16) the topic of deep interest,(17) the topic of deep interest,(18) the topic of deep interest,(19) the topic of deep interest,(18) the topic of deep interest,(19) the topic of deep interest,