Placental Protein−5による婦人科悪性腫瘍の転移抑制効果

胎盘蛋白5抑制妇科恶性肿瘤转移的作用

• 批准号: 09771291
• 负责人: 宮城 悦子
• 金额: $ 1.22万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09771291/
• 关键词: Placental Protein-5; 転移; 婦人科悪性腫瘍; 絨毛癌; 卵巣癌; プロテアーゼ; プロテアーゼインヒビター; 胎盤

1.正常胎盤組織,絨毛性疾患におけるplacental protein-5遺伝子発現とその局在の検討.正常絨毛組織の形成過程にplacental protein-5(PP5)が関与することを明らかにするため,様々な妊娠週数の胎盤におけるPP5の遺伝子発現をNothern blot法により検討した.その結果,PP5は,妊娠全期間の胎盤において著しく強い遺伝子発現を示した.また,PP5産生細胞の局在を明らかにするため,in situ hybridization法による検討を行った結果,PP5 mRNAの発現が,syncytio+rophoblastに特異的に認められることが明らかになった.以上の結果より,PP5が,正常胎盤の形成過程,分化に重要な役割を果たしている可能性が示唆された.絨毛性疾患におけるPP5遺伝子発現については,現在検体を収集しつつ検討を行っている段階であるが,胞状奇胎組織ではPP5遺伝子発現が認められ,絨毛癌由来細胞株ではPP5遺伝子発現が欠如していることより,PP5が絨毛性疾患の悪性進展に何らかの役割を果たしていると考え,研究を進めている.2.卵巣悪性腫瘍におけるPP5遺伝子発現.卵巣腫瘍の進展におけるPP5の関与を明らかにするために,16例の上皮性卵巣悪性腫瘍臨床検体を用いてNothern blot法によりPP5の遺伝子発現を検討した.その結果,PP5遺伝子は16例中13例(81%)と高頻度に発現が認められ,特に進行癌でより高頻度に発現が見られた.トリプシンは,PP5がプロテアーゼインヒビターとしての活性を有し,転移に促進的をに働くと考えられているセリンプロテアーゼである.トリプシンとPP5の遺伝子発現をともに有する検体が,特に進行癌で多く認められたことより,PP5は卵巣腫瘍組織内での過剰なプロテアーゼ活性による組織破壊の抑制,形態維持に対して何らかの役割を果たしているものと考え検討を進めている.

1. Normal fetal tissue, hairy disease and placental protein-5 disease were found in normal fetal tissue and normal fetal tissue. The normal hair tissue formation process placental protein-5 (PP5) test and the normal hair tissue test showed that the number of pregnancies was significantly higher than that of the normal hair tissue. The number of pregnancies was significantly higher than that of the normal hair tissue. The number of pregnant women was significantly higher than that of the normal hair tissue (PP5). The number of pregnant women and the number of pregnancies were detected by Nothern blot. The results showed that the pregnancy rate of the whole pregnancy was significantly higher than that of the PP5. In this paper, the results of the PP5 health care bureau are as follows: the results of the in situ hybridization method, the results of the PP5 mRNA method, the results of the syncytio+rophoblast method, the results, and the results. The above results show that PP5, normal fetal pregnancy formation process, differentiation of important labor to cut off the possibility of pregnancy is instigating the possibility. The disease of human hair is known to be complicated by PP5 disease. Now, in the collection of human body disease, it is necessary to detect the disease, the tissue of cellular ectopic fetus, the tissue of PP5, the disease of hair cancer, the disease of hair, the disease of PP5, the disease of hair, and the progress of hair disease. The research is very advanced. 2. The egg was sexually active, and the PP5 was detected. The clinical data of 16 patients with epithelial oocytes were detected by Nothern blot method. The results showed that PP5 was not detected in 16 patients with epithelial oocyte, and in 16 patients with epithelial oocytes. The results showed that 13 cases (81%) of 16 cases of PP5 were diagnosed with high degree of disease, especially for cancer. In the first place, PP5 has been shown to be active, and the transfer has been promoted in a variety of ways. In this case, you can see that there is a significant increase in the number of cancers in the PP5 system, and that in the tissue of the PP5, the activity of the tissue is inhibited, and the morphology is maintained. The results show that there is a significant improvement in the performance of the system.