Impact of exclusive enteral nutrition on microbiome signatures and function in pediatric Crohn’s disease (P01)

克罗恩病（CD）是特发性炎症性肠病（IBD）的两个主要实体之一。在第二个资助阶段，该串联将指定饮食引起的肠道微生物组变化，以用于单独肠内营养（EEN）治疗儿童克罗恩病的疗效。将使用连续培养、小鼠和猪模型的联合方法来表征een相关特征。在此背景下，膳食纤维在引发疾病复发中的作用将被评估。对治疗反应性和难治性患者的纵向细菌和代谢物分析将被实施，以确定对EEN反应的功能相关微生物组靶点，从而导致保护性合成细菌联合体的发展。本项目的目标是开发一种基于微生物组的儿童乳糜泻患者维持治疗方法。

Crohn’s disease (CD) is one of the two main entities of idiopathic inflammatory bowel disease (IBD). In the second funding phase, the tandem will specify the diet-induced changes in the intestinal microbiome for therapeutic efficacy of exclusive enteral nutrition (EEN) in pediatric Crohn’s disease. EEN-associated signatures will be characterized using the combined approach of continuous culture, gnotobiotic mice and pig models. In this context, the role of dietary fiber in provoking disease relapse will be evaluated. Longitudinal bacterial- and metabolite-profiling of treatment responsive and refractory patients will be implemented to identify functionally relevant microbiome targets in response to EEN, leading to the development of protective synthetic bacterial consortia. The perspective of this project is to develop a microbiome-based maintenance therapy for pediatric CD patients.