Role of NEIL DNA glycosylases in DNA demethylation and base excision repair (03)

我们已经发现两种DNA糖基化酶NEIL 1和NEIL 2在泰特/TDG依赖的DNA去甲基化过程中协调核碱基加工。为了研究Neil DNA糖基化酶在DNA去甲基化中的生理相关性和生物学作用，我们产生了Neil基因的小鼠以及小鼠胚胎干细胞突变体。该项目的目的是鉴定这些突变细胞的分化能力和氧化胞嘧啶的分布，并评估Neil突变小鼠的潜在发育缺陷。

We have found that two DNA glycosylases, NEIL1 and NEIL2, coordinate nucleobase processing during TET/TDG-dependent DNA demethylation. To investigate the physiological relevance and bio-logical role of Neil DNA glycosylases in DNA demethylation we have generated mice as well as mouse embryonic stem cells mutant for Neil genes. The aim of this project is to characterise these mutant cells for their differentiation capacity and their profiles of oxidized cytosine, and to assess potential developmental defects of Neil mutant mice.