Structural mechanism of MRN-ATM in DNA double-strand break signalling and processing (18)

MRN-ATM 在 DNA 双链断裂信号传导和处理中的结构机制 (18)

• 批准号: 418753413
• 金额: --
• 依托单位: Genzentrum - Laboratorium für molekulare Biologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/418753413?language=en
• 关键词: Structural; mechanism; MRN; ATM; DNA

This project aims at elucidating how DNA double-strand breaks are detected, signalled and processed by the Mre11-Rad50-Nbs1-ATM axis. To achieve this, we will use a combination of cryo-electron microscopy, bio-chemistry and cell-based studies. In particular, we will functionally and structurally dissect catalytic and scaffolding roles of MRN in order to provide molecular mechanisms for its poorly understood, pleiotropic func-tions in double-strand break repair and chromatin architecture.

该项目旨在阐明DNA双链断裂是如何通过Mre 11-Rad 50-Nbs 1-ATM轴检测、发出信号和处理的。为了实现这一目标，我们将使用冷冻电子显微镜，生物化学和基于细胞的研究相结合。特别是，我们将在功能和结构上剖析MRN的催化和支架作用，以便为其在双链断裂修复和染色质结构中的多效性功能提供分子机制。