膵ランゲルハンス島移植における選択的肝動脈注入法の検討

选择性肝动脉注射法在胰岛移植中的探讨

• 批准号: 11770717
• 负责人: 衛藤 謙
• 金额: $ 1.41万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11770717/
• 关键词: 膵ランゲルハンス島; 選択的肝動脈注入法; セルディンガー法

糖尿病の治療に膵ランゲルハンス島(ラ島)移植があり,昨年度より,セルディンガー法を利用したラ島の選択的肝動脈注入法を試みている.昨年度は,血糖のコントロールが不十分でラ島数の不足が考えられた.今年度はdonorを2匹とし,ラ島数を増やし移植を試みた.1.膵臓全摘出による糖尿病犬の作成およびラ島の単離昨年度と同様に犬(体重15〜20Kg)を使用し,全身麻酔下で開腹した.十二指腸は温存し,膵臓全摘出術を施行.次に摘出した膵臓からラ島を単離した.保存液から膵臓を取り出し,膵管にHBSSで溶解したコラゲナーゼを注入し,膵臓を膨化させた.その後,膵臓を細切し,HBSS液で10分間37℃にてdigestionする.次に60分間程度冷温でdigestionを行う.最後にFicoll液で濃度勾配法を用いてラ島のみを単離した.ラ島はRPMI1640培地に入れ,5%CO_2,37℃の条件下で培養した.2.ラ島の移植膵臓全摘出し3〜4週後,糖尿病(血糖が300mg/dL以上)になった犬をrecipientとした.全身麻酔下で透視を用い犬の右鼠径部からRHカテーテルをセルディンガー法で挿入し,ガイドワイヤーを使用して選択的に肝動脈にRHカテーテルを挿入した.培養後,凍結保存していたラ島を溶解し,生理食塩水3ccに入れ,RHカテーテル内を通し肝臓内にラ島を移植した.ラ島数は300000〜400000islet equivalents(IEQ)とし,昨年度より数を増やし移植を試みた.しかし,移植の効果を認めるまでには至っておらず,今後さらに検討していく予定である.

Diabetes treatment in the treatment of diabetes mellitus should be based on the selection of liver artery infusion method. Last year, the blood sugar level was not very high. This year, the number of donor dogs increased and the number of transplants was tested. 1. The number of diabetic dogs was completely removed. 2. The number of diabetic dogs was tested. 3. The number of diabetic dogs (weighing 15 ~ 20Kg) was tested. Duodenal warm, full extraction operation. The next day, I went to the hospital. Preservative solution is extracted, HBSS is dissolved in the tube, and the solution is injected into the tube. After that, the mixture was finely cut,HBSS solution was digested at 37℃ for 10 minutes. The next 60 minutes cold temperature digestion line. Finally, Ficoll solution concentration matching method is used to determine the concentration of Ficoll solution. RPMI1640 was cultured in 5%CO_2 at 37 ℃. 2. After 3 ~ 4 weeks of transplantation, diabetic dogs (blood glucose> 300mg/dL) were treated with RPMI1640. Under general anesthesia, the right part of the rat's diameter was exposed to RH and the right part of the rat was exposed to RH and the right part of the rat was exposed to RH. After culture, frozen preservation, dissolution, physiological water 3cc into the RH temperature range, open to liver tissue, transplantation. The number of islands is 300,000 ~ 400,000 island equivalents(IEQ), and the number of transplant attempts is increasing. The results of transplantation are recognized and discussed in the future.