脊髄慢性圧迫による前角細胞の遺伝子発現とアポトーシス

慢性脊髓压迫导致前角细胞的基因表达和凋亡

• 批准号: 11770769
• 负责人: 荻野 雅宏
• 金额: $ 1.47万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11770769/
• 关键词: anterior column; apoptosis; chronic compression; neuronal loss; spinal cord; apoptsis

Wister rat(体重250・300g)を用い、全身麻酔の下、第5・6頸椎椎弓下に膨張性ポリマーを挿入して局所脊髄圧迫を作成した。対照として、ポリマーを通過させただけで留置しないシャム手術群を設けた。術前より自発性運動量を回転式運動量測定ケージで、また強制運動能力をトレッドミルならびに傾斜姿勢維持測定板にてそれぞれ計測した。術後はポリマー挿入操作による急性脊髄損傷のないことを確認した上で、3週ごとに上記の運動能を計測した。動物は術後3週、6週、9週、12週、24週の各時点に屠殺した。経心灌流固定ののち全脊髄を摘出し、スライサーにて圧迫部位を含む連続切片を作成した。免疫組織化学的手法により、代表的即初期遺伝子産物でるc-Fos,c-Junの産生を検討した。自発運動量が24週の観察期間中にシャム群と圧迫群との間に差を認めぬこと、強制運動能力が18週より24週まで進行性に低下することは過年度の結果と相違なかった。c-Fosならびにc-Jun免疫染色による検討によっても、形態学的変化すなわち前角の運動ニューロン密度の低下に先だってapoptotic changeが現れていることが示唆された。過年度の成果を併せて考慮すると、研究者らが用いているモデルにおいて、脊髄慢性圧迫による前角細胞脱落と虚血後の遅発性神経細胞死とがcommon pathwayを持つ可能性が示唆された。今後は、同モデルを用いて脊髄血流量の測定を行う予定としている。

Wister rat (body weight 250,300g) was used under general anaesthesia, and was forced into the spinal cord under the spinal arch of the 5th and 6th vertebrae. According to the information, please check the setting of the mobile phone group by setting it up. In front of you, you can use your own motion measurement to determine your performance, and you need to force your ability to maintain the oblique attitude of the measuring board. After that, you need to know that you are in the middle of the operation. After that, you have to make sure that you are in the middle of the operation. After that, you have to make sure that you are in the middle of the operation. The animals were slaughtered at 3, 6, 9, 12 and 24 hours after operation. The whole spine was removed by cardiac perfusion, and the forced part of the whole spine was made by linking slices. The immuno-histochemical technique, which represents the early stage of the disease, is to give birth to the baby baby by using the method of immuno-histochemistry. During the 24-hour surveillance period, the local population is forced to make a difference, and the ability to exercise is 18-year-old and 24-year-old. The annual results show that there is no significant difference between the two groups. C-Fos was detected by c-Jun immunostaining, and the morphology was detected in the front corner. The density was low and the density was low. Apoptotic change immunostaining was used to show that it was instigated. According to the results of the year, the researchers showed that the possibility of the death of the sexual goddess cell was abetted by the possibility that the spinal cord was chronically forced to fall off the anterior horn of the spinal cord, and after the deficiency of blood. The possibility of common pathway holding on to the possibility of death. In the future, we will use the spinal blood flow to determine the predetermined blood flow.