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Selective plane illumination microscope (SPIM) with IT-equipment

配备 IT 设备的选择性平面照明显微镜 (SPIM)

基本信息

批准号：
420665888
负责人：
金额：
--
依托单位：
Heinrich-Heine-Universität Düsseldorf
依托单位国家：
德国
项目类别：
Major Research Instrumentation
财政年份：
2019
资助国家：
德国
起止时间：
2018-12-31 至无数据
项目状态：
未结题

来源：
https://gepris.dfg.de/gepris/projekt/420665888?language=en
关键词：
Selective plane illumination microscope SPIM

项目摘要

We deploy in vivo confocal imaging techniques in two major areas: (i) Transport processes in plants, and (ii) biosensors for small molecules and biochemical/biophysical processes. We systematically identify proteins that are responsible for the uptake and release of ions and metabolites and characterize their roles in the physiology of plants. To characterize the subcellular localization and regulation of the transport proteins, we use confocal imaging technology. We also develop and deploy genetically encoded fluorescent biosensors for ions, metabolites and signaling molecules in vivo. We pioneered the development of transporter activity sensors. To be able to record sensor output, we require highly sensitive and quantitative fluorescence imaging technologies. In my previous lab at Stanford, we used both spinning disk and selective plane illumination microscopy to study transporter regulation and biosensor responses. This application requests funds for a set of two fluorescence microscopes as well as an image data storage and processing system. After careful testing and evaluation of multiple instruments, we would like to acquire: [...] The systems will not only be used by our team, but integrated into CAi (Center for Advanced Imaging) in Düsseldorf, and thus be accessible to a wide range of scientists. Moreover we have included specific descriptions of the needs for 5 additional teams at the University and the Clinics.

我们在两个主要领域部署了体内共聚焦成像技术：(I)植物中的运输过程，以及(Ii)用于小分子和生化/生物物理过程的生物传感器。我们系统地识别了负责离子和代谢物的吸收和释放的蛋白质，并描述了它们在植物生理中的作用。为了表征运输蛋白的亚细胞定位和调控，我们使用了共聚焦成像技术。我们还在体内开发和部署针对离子、代谢物和信号分子的基因编码荧光生物传感器。我们率先开发了转运蛋白活动传感器。为了能够记录传感器的输出，我们需要高灵敏度和定量的荧光成像技术。在我之前在斯坦福大学的实验室里，我们使用旋转圆盘和选择性平面照明显微镜来研究转运蛋白调节和生物传感器响应。这项申请申请了一套两台荧光显微镜以及一个图像数据存储和处理系统的资金。经过对多种仪器的仔细测试和评估，我们希望获得：[...]这些系统不仅将被我们的团队使用，而且还将集成到位于杜塞尔多夫的高级成像中心，从而为广泛的科学家所使用。此外，我们还对大学和诊所另外5个小组的需要作了具体说明。