Function, mechanism and structural basis for the regulation of gene expression by small regulatory RNAs (sRNAs) in γ-proteobacteria (B17*)

γ-变形菌 (B17*) 中小调节 RNA (sRNA) 调节基因表达的功能、机制和结构基础

• 批准号: 423465556
• 金额: --
• 依托单位: Institut für Molekulare Biowissenschaften
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/423465556?language=en
• 关键词: Function; mechanism; structural; basis; regulation

In bacteria small regulatory RNAs (sRNAs) play an important role in the regulation of protein expression and facilitate the rapid adaptation of the bacterial metabolism to environmental changes. Many bacterial sRNAs work in concert with the RNA-binding proteins Hfq and ProQ. We showed that Hfq- and ProQ-mediated sRNA-based mechanisms strongly influence the biosynthesis of secondary metabolites in certain -proteobacteria and solved the structure of a minimal ProQ homolog. The aim of this project is to delineate the molecular basis for sRNA-based regulatory mechanisms in these bacteria by combining transcriptomic methods, in vivo mutagenesis, in vitro biochemical and biophysical experiments with structural biology approaches

在细菌中，小调节RNA(SRNAs)在调节蛋白质表达和促进细菌新陈代谢对环境变化的快速适应中发挥着重要作用。许多细菌sRNA与RNA结合蛋白Hfq和ProQ协同工作。我们发现HfQ和ProQ介导的基于SRNA的机制强烈影响某些蛋白细菌中次生代谢产物的生物合成，并解决了最小ProQ同源物的结构。这个项目的目的是通过结合转录方法、体内诱变、体外生化和生物物理实验以及结构生物学方法来描述这些细菌中基于srna的调控机制的分子基础。