Role of autoinducer 2 signalling in shaping spatio-temporal dynamics and host-microbe interactions of the gut microbiota

自诱导剂2信号传导在塑造肠道微生物群的时空动态和宿主-微生物相互作用中的作用

• 批准号: 424173447
• 负责人: Dr. Leanid Laganenka
• 金额: --
• 依托单位: Institut für Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/424173447?language=en
• 关键词: Role; autoinducer; signalling; shaping; spatio

Gut microbiome represents a dynamic microbial community that is shaped by a range of microbe-microbe and host-microbe interactions. The role of interspecies communication in development of such mixed communities is becoming apparent, although our understanding of its mechanisms in vivo remains limited. The universal signalling molecule autoinducer 2 (AI-2) is known to regulate cell-density-dependent phenotypes of many bacteria and is thus considered a potential interspecies communication signal. However, the role of AI-2 in the establishment and shaping of complex multispecies communities such as that in the gut is not well understood.Here, using E. coli and S. Typhimurium as model organisms, the role of AI-2 signalling in murine gut colonization, interspecies communities formation and infection will be investigated. Additionally, the impact of AI-2 on shaping biogeography and interactions between gut microbiota and the host immune system will be addressed. This will shed light on physiological relevance of AI-2 signalling in gut microbial communities and lay foundation for step-by-step understanding of how microbial communities are formed in the gut, how bacteria within them interact with each other and with the host.

肠道微生物群落是由一系列微生物-微生物和宿主-微生物相互作用形成的动态微生物群落。种间通讯在这种混合群落发育中的作用日益明显，尽管我们对其体内机制的了解仍然有限。已知通用信号分子自动诱导物2（AI-2）调节许多细菌的细胞密度依赖性表型，因此被认为是潜在的种间通信信号。然而，AI-2在复杂的多物种群落（如肠道群落）的建立和形成中的作用还没有得到很好的理解。coli和革兰氏阳性菌S.以鼠伤寒为模型生物，研究AI-2信号转导在小鼠肠道定植、种间群落形成和感染中的作用。此外，AI-2对形成生物地理学的影响以及肠道菌群和宿主免疫系统之间的相互作用也将得到阐述。这将阐明AI-2信号转导在肠道微生物群落中的生理相关性，并为逐步了解肠道微生物群落是如何形成的，其中的细菌如何相互作用以及与宿主的相互作用奠定基础。