Spatial and temporal cellular assembly of the glycopeptide biosynthetic complex (B01+)

糖肽生物合成复合物的空间和时间细胞组装（B01）

• 批准号: 425871802
• 金额: --
• 依托单位: Lehrstuhl für Mikrobiologie / Biotechnologie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/425871802?language=en
• 关键词: Spatial; temporal; cellular; assembly; glycopeptide

Balhimycin, a glycopeptide synthesized by Amycolatopsis balhimycina, is a heptapeptide, which is modified by cyclization, methylation, halogenation, and glycosylation. Using genetics and biochemistry, its biosynthetic steps have been elucidated indicating the occurrence of a large multi-enzyme complex that catalyzes amino acid assembly as well as various modifications. Whereas the chronology of the reactions could be defined, nothing is known about the positioning and dynamics of such a super-complex. Protein interaction studies and state-of-the-art microscopy will localize the complexes in the cell, identify further putative interaction partners, and define the temporal assembly of the complex.

巴利菌素是由巴利霉菌合成的糖肽，是经环化、甲基化、卤化和糖基化修饰而成的七肽。利用遗传学和生物化学，其生物合成步骤已被阐明，表明存在一个大的多酶复合体，催化氨基酸组装和各种修饰。虽然可以定义这些反应的年表，但人们对这种超级复合体的位置和动力学一无所知。蛋白质相互作用研究和最先进的显微镜将定位细胞中的复合体，确定进一步的假定相互作用伙伴，并定义复合体的时间组装。