Nanoscale organization of adaptor scaffolds involved in polarized cargo sorting at the trans-Golgi network (TGN) (A25*)

参与跨高尔基体网络 (TGN) 极化货物分选的适配器支架的纳米级组织 (A25*)

• 批准号: 425957988
• 金额: --
• 依托单位: Institut für Chemie und Biochemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/425957988?language=en
• 关键词: Nanoscale; organization; adaptor; scaffolds; involved

In project A25 we will investigate the sub-compartmental nanodomain organization of the trans-Golgi network (TGN) that likely underlies the spatio-temporally controlled sorting of distinct cargos to different organelles or plasma membrane domains. Harnessing CRISPR/Cas9 knock-in cell lines for live-cell super-resolution imaging and acute protein degradation strategies, we aim to dissect the functional role of adaptor-based membrane scaffolding proteins in TGN nanodomain organization to segregate and sort distinct membrane cargoes in polarized epithelial cells.

在项目A25中，我们将研究trans-Golgi网络（TGN）的亚隔室纳米结构域组织，这可能是时空控制的不同货物分选到不同细胞器或质膜结构域的基础。利用CRISPR/Cas9敲入细胞系进行活细胞超分辨率成像和急性蛋白质降解策略，我们的目标是剖析基于衔接子的膜支架蛋白在TGN纳米结构域组织中的功能作用，以分离和分选极化上皮细胞中的不同膜货物。