Modelling mechanisms of membrane shaping, fusion, and fission in endo- and exocytosis by protein scaffolds (A26*)

我们将使用膜弹性理论来解决膜重构的关键机制：由BAR家族蛋白与动力蛋白和网格蛋白接头共同介导的网格蛋白介导的内吞作用；肌动蛋白-肌球蛋白复合物介导的突触网格蛋白独立内吞作用和突触前活性区由支架蛋白介导的突触囊泡胞吐。目标是为实验项目（A01）、（A07）、（A12）、（Z02）、（A03）、（A05）和（A06）提供可测试的预测，并补充（A04）进行的数值模拟结果。

We will use the theory of membrane elasticity to address key mechanisms of membrane remodelling in: clathrin-mediated endocytosis mediated by BAR family proteins in conjunction with dynamin and clathrin adaptors; clathrin independent endocytosis at synapses mediated by actin-myosin complexes; and synaptic vesicle exocytosis in the presynaptic active zone mediated by scaffolding proteins. The goal is to provide testable predictions for the experimental projects (A01), (A07), (A12), (Z02), (A03), (A05), and (A06) and complement the results of numerical simulations performed by (A04).