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カザフスタン低線量慢性被ばく患者の肺癌発生:ゲノム不安定性亢進関与の証明

哈萨克斯坦长期暴露于低剂量辐射的患者罹患肺癌：基因组不稳定性增加的证据

基本信息

批准号：
19659091
负责人：
原敦子
金额：
$ 1.98万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Exploratory Research
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2008
项目状态：
已结题

项目摘要

カザフスタン共和国セミパラチンスク核実験場周辺での低線量域放射線慢性・頻回被ばくとがんとの関連についての検討を、本年度も引き続き以下の内容で施行した。1.東カザフスタン州およびパブロダール州における被ばく者等に関する情報入手作業を行い、結果をデータベース化した。(1)被ばく者・非被ばく者情報収集(居住歴・死亡情報・疾患罹患歴など)調査対象者数約11万人。(2)被ばく者の推定被ばく線量の計算(ロシア共和国提示計算式を使用)。解析の結果、各疾患死亡者における被ばく相対リスク(被ばく者と非被ばく者間比)は循環器疾患で有意差を認めたが、癌全般については有意差は見られなかった。2.平成20年11月、パブロダール州がんセンターに出張、被ばく者・非被ばく者肺がん症例に関する情報交換を行った。(1)現地の慢性被ばく推定がん患者のリストを入手。(2)当該症例のパラフィンブロックとスライドガラスを抽出、借用((1)被ばく地域居住歴のある肺がん症例3例。(2)コントロールとして現地手術症例(胃がん37例、食道がん5例、肺がん5例、および無作為抽出20症例。)3.借用症例を用いゲノム不安定性・異常の検索を行った。まずmicrodissection法により腫瘍細胞を分離採取・DNA抽出し、主に17番染色体マーカーにおけるMSI/LOHをMultiplex PCR-Gene Scan法で検索した。しかし借用ブロックは作成時点での固定不良のためDNA抽出量が少なく検出が不安定であり、現在も引き続き検索を行っている。4,借用標本を用いカザフスタン病理診断精度の確認作業を行ったが、日本との診断一致率は90%以上であり高い診断精度が認められた。また現地への病理診断支援を目的としてインターネット上のテレコンサルテーションシステムの開発運用を行い、結果を日本病理学会総会で発表した。

The Republic of China is responsible for the development of low-line radiation in the area of low-line radiation. This year, the following contents will be implemented. 1. If you are in a bad situation, such as those who are in the market, and so on, start the operation operation, and the results will result in the operation of the operation. The main results are as follows: (1) the number of people who are not pregnant (living in death, illness, illness and illness) is about 110000. (2) the presumption of the victim is calculated by the quantity calculation (the Republic prompt calculation formula is used). According to the analysis of the results, the patients who died of each disease were compared with each other (those who died were compared with those who were not). The patients with environmental diseases were intentionally affected by environmental diseases, and the patients with cancer were not affected by cancer. two。 In November of the 20th year, Pingcheng was not affected by the disease of the lungs of those who were not affected by the disease. In November of the 20th year, there was a case of lung disease. The main results are as follows: (1) the chronic disease is presumed to be the starting point of the patients. (2) when the case of the disease was drawn out and borrowed (1) there were 3 cases of lung disease due to geographical residence. (2) there were 37 cases of gastroenteritis, 5 cases of esophageal dysphagia, 5 cases of pulmonary dysphagia and 20 cases of dysphagia.) 3. To borrow an example of the disease, we often use it to determine the nature of the disease. The cells were isolated by microdissection method, and the chromosomes were extracted by DNA extraction. The chromosomes of 17 times were isolated by Multiplex PCR-Gene Scan method. In order to reduce the amount of instability caused by poor fixation, the amount of DNA is not enough to cause instability, and now it is necessary to make sure that it is not safe to do so. 4. Use the tag book to confirm that the accuracy of the operation is higher than that of the Japanese, and the accuracy of the accuracy is higher than 90%. The purpose of this study is to improve the safety and safety of local pathology. the results show that the association of Japanese Pathology has been listed in the following table.