Developing the Dunnart as a Model Species for Marsupial Research

将邓纳特发展为有袋动物研究的模式物种

• 批准号: DP160103683
• 负责人: Prof Andrew Pask
• 金额: $ 48.16万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160103683
• 关键词: Developing; Dunnart; Model; Species; Marsupial

The project aims to develop a marsupial model capable of genome manipulations to take our understanding of marsupial biology to the next level. In doing so, the project would produce the first comprehensive transcriptome data defining early cell lineage specification in a marsupial. Combined with similar data from mouse and human, it would enable us to examine diversity in early mammals. In addition, it would identify cohorts of genes with fundamental roles in differentiation of the earliest cell lineages: trophoblast, pluriblast and hypoblast. The project may identify maternally localised transcripts with a marsupial-specific role in trophoblast–pluriblast specification, giving new insights into the fundamental pathways maintaining pluripotency in mammals and the evolution of the mammalian genome.

该项目旨在开发一种能够进行基因组操作的有袋类动物模型，将我们对有袋类生物学的理解提升到一个新的水平。通过这样做，该项目将产生第一个全面的转录组数据，定义有袋类动物的早期细胞谱系规范。结合来自老鼠和人类的类似数据，它将使我们能够检查早期哺乳动物的多样性。此外，它还将确定在最早的细胞谱系：滋养层细胞、多核细胞和低分化细胞分化过程中起基础作用的基因群。该项目可能识别母体定位的转录本，在有袋类动物的滋养细胞-多母细胞指定中发挥特定的作用，为维持哺乳动物多能性的基本途径和哺乳动物基因组的进化提供新的见解。