Structure and Function of Proteins Involved in the Formation of Supra-Structure of Chromatin in Animals

参与动物染色质超结构形成的蛋白质的结构和功能

• 批准号: 01470118
• 负责人: MIZUNO Shigeki
• 金额: $ 4.42万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01470118/
• 关键词: W chromosome; heterochromatin; repetitive DNA; bent DNA; DNA-binding protein; fibroin H-chain gene; silk gland nuclei; nucleosome; ヘテロクロマチン; 性染色体; W染色体(ニワトリの); 湾曲(bent)DNA; W-protein; DNaseIフィットプリント法; クロマチン

1) Structure of W-heterochromatic body of chicken : About 60% of DNA in the chicken W chromosome has been shown to consist of XhoI-family repetitive sequences. It was found that 10-30% of the DNA in the W chromosome consisted of another repetitive family, EcoRI-family. The 1.2kb repeating unit of the EcoRI-family was cloned and sequenced. Although overall sequence similarity was 68%, their sequence organizations, high level methylation in the genome, and strongly bent conformation were vety similar each other. Protein components showing high affinity binding to these two bent-repetitive sequences were found in the chicken nuclei and were purified partially.2) Inactive chromatin structure of fibroin H-chain gene : Transcription of fibroin H-chain gene is inactive in the 5th-instar middle silk gland and in the 4th-molting stage posterior silk gland. When isolated nuclei from these inactive tissues were digested with DNaseI at relatively high concentration, the H-chain gene chromatin produced regular patterns of DNA fragments, which was most likely caused by phased nuclesomal arrangement along the repetitive DNA sequences corresponding to the "crystalline" regions in fibroin H-chain.

1)鸡W异染色质体的结构：鸡W染色体中约60%的DNA由XhoI家族重复序列组成。发现W染色体上10-30%的DNA由另一个重复家族EcoR Ⅰ家族组成。克隆了EcoR Ⅰ家族的1.2kb重复单位并测序。虽然它们的序列相似性为68%，但它们的序列组织、基因组中的高水平甲基化和强弯曲构象彼此非常相似。2）丝素H链基因的失活染色质结构：丝素H链基因在5龄中期丝腺和4龄蜕皮期后部丝腺中的转录是失活的。当从这些失活组织中分离的细胞核用相对高浓度的DNaseI消化时，H-链基因染色质产生规则的DNA片段图案，这很可能是由与丝心蛋白H-链中的“结晶”区域相对应的重复DNA序列沿沿着的核小体排列引起的。

项目成果

水野重樹(分担執等): "シリ-ズ分子生物学の進歩6 染色体と細胞周期(柳田充弘,西本毅治,掘田康雄編)" 丸善株式会社, 266 (1989)

水野茂树（合著者）：《分子生物学系列进展 6：染色体和细胞周期（柳田光宏、西本武治、堀田康夫编辑）》丸善株式会社，266（1989）

H.Saitoh: "Presence of femaleーspecific bentーrepetitive DNA sequences in the genomes of turkey and pheasant and their interactions with Wーprotein of chicken" Chromosoma. 98. 250-258 (1989)

H. Saitoh：“火鸡和雉鸡基因组中雌性特异性弯曲重复 DNA 序列的存在及其与鸡 W 蛋白的相互作用”《染色体》98. 250-258 (1989)。

S. Mizuno: Japan Sci. Soc. Press, Tokyo and Springer-Verlag, Berlin. New aspects of the genetics of molecular evolution (eds. M. Kimura and N. Takahata),213-226 (1991)

S. Mizuno：日本科学。

Y.Saitoh: "Occupancy of the majority of DNA in the chicken W chromosome by bentーrepetitive DNA sequences" Chromosoma. (1991)

Y.Saitoh：“Bentorepetitive DNA 序列占据鸡 W 染色体中的大部分 DNA”Chromosoma（1991）。

Y. Saitoh: "Occupancy of the majority of DNA in the chicken W chromosome by bent-repetitive DNA sequences" Chromosoma. (1991)

Y. Saitoh：“弯曲重复 DNA 序列占据鸡 W 染色体中的大部分 DNA”染色体。