Production of D-pantothenate with Microbial Enzymes

利用微生物酶生产D-泛酸

• 批准号: 01470124
• 负责人: YAMADA Hideaki
• 金额: $ 4.86万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01470124/
• 关键词: D-Pantothenic acid; D-Pantoyl lactone; Carbonyl reductase; Stereospecific reduction; D-パントテン酸; D-パントイルラクトン; カルボニルレダクタ-ゼ

New NADPH-dependent carbonyl reductases which catalyzes stereospecific reductions of carbonyl compounds have been purified from various microorganisms and characterixed. The enzyme "polyketone reductase" from Candida parapsilosis specifically catalyzes reduction of various prochiral diketones, yielding the corresponding (R) - hydroxyketones. Aldehyde reductase from Sporobolomyces salmonicolor and carbonyl reductase from Candida macedoniensis catalyze stereospecific reduction of various aliphatic and aromatic ketones and aldehydes. Ketopantoic acid reductase from Pseudomonas maltophilia and an FMN-dependent alcohol dehydrogenase from Nocardia asteroides, catalyze reduction of ketopantoic acid to D-pantoic acid and oxidation of L-pantoyl lactone to ketopantoyl lactone, respectively. These enzymes were successfully applied to the synthesis of chiral intermediates for the syntesis of D-pantothenate.

新的NADPH依赖性羰基还原酶催化了从各种微生物中纯化了羰基化合物的立体特异性降低并具有象征性。来自念珠菌的降低酶的酶“多酮还原酶”特异性催化了各种甲二酮的降低，得出相应的（R） - 羟基酮酮。来自孢子菌的孢子菌和羰基还原酶的醛还原酶来自念珠菌的马其顿念珠菌催化了各种脂肪族和芳香族酮和醛的立体特异性减少。来自假单胞菌一个友善的酮症还原酶和来自诺卡氏菌的Atteroides的FMN依赖性酒精脱氢酶，分别将酮症酸还原为D-Pantoic酸，并将L-Pantoyl lactoyl lactoyl lactone lactone lactonel lactoyl lactoyl lactonoyl lactonoyl lactoyl lactoyl lactoyl氧化。这些酶成功地应用于手性中间体的合成D-苯甲酸苯甲酸酯。

项目成果

Michihiko Kataoka: "Novel enzymatic production of Dー(ー)ーpantoyl lactone through the stereopecific reduction of ketopantoic acid" Agric.Biol.Chem.54. 177-182 (1990)

Michihiko Kataoka：“通过酮泛解酸立体定向还原 Dー(ー)ー泛酰内酯的新型酶法生产”Agric.Biol.Chem.54 (1990)。

Hiroyuki Hata: "Ketopantoyl lactone reductase is a conjugated polyketone reductase" FEMS Microbiol.Lett.58. 87-90 (1989)

Hiroyuki Hata：“酮泛酰内酯还原酶是一种共轭聚酮还原酶”FEMS Microbiol.Lett.58。

M. Kataoka et al.: "Stereospecific reduction of ethyl 2'-ketopantothenate eith microbial cells as a catalyst" Appl. Environ. Microbiol.56. 3595-3597 (1990)

M. Kataoka 等人：“以微生物细胞作为催化剂对 2-酮泛酸乙酯进行立体定向还原”Appl。

Hiroyuki Hata: "Ketopantoyl lactone reducatse is a conjugated polyketone reducatse" FEMS Microbiol.Lett.58. 87-90 (1989)

Hiroyuki Hata：“酮泛酰内酯还原酶是一种共轭聚酮还原酶”FEMS Microbiol.Lett.58。

Michihiko Kataoka: "Microbial production of chiral pantothenonitrile through stereospecific reduction of 2'ーketopantothenonitrile" Biotechnol.Lett.12. 357-360 (1990)

Michihiko Kataoka：“通过 2-酮泛腈的立体定向还原来微生物生产手性泛腈”Biotechnol.Lett.12 (1990)。