Production of D-pantothenate with Microbial Enzymes

利用微生物酶生产D-泛酸

• 批准号: 01470124
• 负责人: YAMADA Hideaki
• 金额: $ 4.86万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01470124/
• 关键词: D-Pantothenic acid; D-Pantoyl lactone; Carbonyl reductase; Stereospecific reduction; D-パントテン酸; D-パントイルラクトン; カルボニルレダクタ-ゼ

New NADPH-dependent carbonyl reductases which catalyzes stereospecific reductions of carbonyl compounds have been purified from various microorganisms and characterixed. The enzyme "polyketone reductase" from Candida parapsilosis specifically catalyzes reduction of various prochiral diketones, yielding the corresponding (R) - hydroxyketones. Aldehyde reductase from Sporobolomyces salmonicolor and carbonyl reductase from Candida macedoniensis catalyze stereospecific reduction of various aliphatic and aromatic ketones and aldehydes. Ketopantoic acid reductase from Pseudomonas maltophilia and an FMN-dependent alcohol dehydrogenase from Nocardia asteroides, catalyze reduction of ketopantoic acid to D-pantoic acid and oxidation of L-pantoyl lactone to ketopantoyl lactone, respectively. These enzymes were successfully applied to the synthesis of chiral intermediates for the syntesis of D-pantothenate.

新的NADPH依赖性羰基还原酶催化羰基化合物的立体特异性还原，已从各种微生物中纯化并表征。来自近平滑假丝酵母的酶“聚酮还原酶”特异性催化各种前手性二酮的还原，产生相应的（R）-羟基酮。来自Sporobolomyces salmonicolor的醛还原酶和来自Candida macedoniensis的羰基还原酶催化各种脂肪族和芳香族酮和醛的立体特异性还原。来自嗜麦芽假单胞菌的酮泛解酸还原酶和来自星状诺卡氏菌的FMN依赖性醇脱氢酶分别催化酮泛解酸还原为D-泛解酸和L-泛酰内酯氧化为酮泛酰内酯。这些酶已成功地应用于D-泛酸的手性中间体的合成。

项目成果

Michihiko Kataoka: "Novel enzymatic production of Dー(ー)ーpantoyl lactone through the stereopecific reduction of ketopantoic acid" Agric.Biol.Chem.54. 177-182 (1990)

Michihiko Kataoka：“通过酮泛解酸立体定向还原 Dー(ー)ー泛酰内酯的新型酶法生产”Agric.Biol.Chem.54 (1990)。

Hiroyuki Hata: "Ketopantoyl lactone reductase is a conjugated polyketone reductase" FEMS Microbiol.Lett.58. 87-90 (1989)

Hiroyuki Hata：“酮泛酰内酯还原酶是一种共轭聚酮还原酶”FEMS Microbiol.Lett.58。

M. Kataoka et al.: "Stereospecific reduction of ethyl 2'-ketopantothenate eith microbial cells as a catalyst" Appl. Environ. Microbiol.56. 3595-3597 (1990)

M. Kataoka 等人：“以微生物细胞作为催化剂对 2-酮泛酸乙酯进行立体定向还原”Appl。

Hiroyuki Hata: "Ketopantoyl lactone reducatse is a conjugated polyketone reducatse" FEMS Microbiol.Lett.58. 87-90 (1989)

Hiroyuki Hata：“酮泛酰内酯还原酶是一种共轭聚酮还原酶”FEMS Microbiol.Lett.58。

H. Hata et al.: "Ketopantoyl lactone reducatse is a conjugated polyketone reductase" FEMS Microbiol. Lett.58. 87-90 (1989)

H. Hata 等人：“酮泛酰内酯还原酶是一种共轭聚酮还原酶”FEMS Microbiol。