Mechanism of impaired rheology of aged erythrocytes in relation to the determinants of life span

衰老红细胞流变学受损与寿命决定因素相关的机制

• 批准号: 01480115
• 负责人: SHIGA Takeshi
• 金额: $ 3.52万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480115/
• 关键词: Erythrocytes,; Life span,; Aging,; 2.3-DPG,; ATP,; Energy metabolism,; Deformability,; Rheology

The life span of human erythrocytes in blood stream depends mainly on the easiness of their filtrability through spleen and on the adhesivity, thus on the cellular deformability. The present studies concerns with the deformability of the in vivo-aged erythrocytes.1. A novel sensitive method for detecting a subtle decrease in cellular deformability is developed, which measures the deformation response in oscillatory shear flow. The frequency response of the aged erythrocytes begins to decrease at ca. 2 Hz at 25 C, while that of young erythrocytes does not at above 3 Hz. The main cause is suggested to be due to the increased intracellular viscosity.2. The decreased energy metabolism in aged erythrocytes has long been said to cause the decrease of the deformability through some change in the dynamics of cytoskeletal structure, without quantitative studies. The present experiments show that the decrease of adenylates, ATP and 2, 3-DPG found in the aged erythrocytes does not affect the cellular deformability. On contrary, the artificially increased 2, 3-DPG impairs the deformability perhaps due to the increased intracellular viscosity.

人红细胞在血液中的寿命主要取决于其通过脾脏的易滤性和粘附性，从而取决于细胞的变形能力。目前的研究关注的是体内老化红细胞的变形能力。本文提出了一种检测细胞变形能力细微下降的灵敏方法，用于测量振荡剪切流中的变形响应。在25℃时，老龄红细胞的频率响应在约2 Hz时开始下降，而年轻红细胞的频率响应在3 Hz以上时不下降。主要原因是细胞内黏度增高。长期以来，人们一直认为衰老红细胞能量代谢的降低是通过细胞骨架结构的动力学变化导致其变形能力的降低，但尚无定量研究。本实验表明，衰老红细胞中腺苷酸、ATP和2,3 - dpg的减少不影响细胞的变形能力。相反，人为增加的2,3 - dpg可能由于细胞内粘度的增加而损害了其变形能力。

项目成果

Shiga,T.,& Kon,K.: "Erythrocyte rheology." Crit.Rev.Oncol.Hematol.10. 9-48 (1990)

滋贺县，T.，

Shiga, T., & Nakajima, T.: "Decreased frequency response of oscillatory deformation of human aged erythrocytes." Studia Biophysica. 134. 167-172 (1989)

志贺，T.，

Shiga,T.,& Nakajima,T.: "Decreased frequency response of oscillatory deformation of human aged erythrocytes." Studia Biophysica. 134. 167-172 (1989)

滋贺县，T.，

Seike,M.et al: "Acceleration of Erythrocyte Aggregation with In Vivo Aging." Clin.Hemorheol.9. 909-922 (1989)

Seike,M.等人：“体内衰老加速红细胞聚集”。

Seike, M., & Shiga, T.: "Acceleration of erythrocyte aggregation with in vivo aging." Clin. Hemorheologe. 9. 909-922 (1989)

塞克，M.，