Developmental Endocrinology and Treatment of Ovarian Cysts in Cattle

牛卵巢囊肿的发育内分泌学和治疗

• 批准号: 01480106
• 负责人: MORI Junichi
• 金额: $ 2.43万
• 依托单位: University of Osaka Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480106/
• 关键词: Cattle; Ovarian Cyst; Hormone Receptors; Hypothalamus; Anterior Pituitary; Follicular Epithelial Cell; Sex Hormones; Cortisol; 視床下部正中隆起; 性腺刺激ホルモン放出ホルモン; 性腺刺激ホルモン; 性ステロイドホルモン; 卵胞中ホルモン

Developmental endocrinology and treatment of ovarian cysts in cattle were examined, and the following results were obtained.1. Cows with ovarian cysts did not have remarkable abnormality in numbers and affinities of gonadotropin-releasing hormone (GnRH) receptors of the anterior pituitary.2. Numbers of gonadotropin receptors and concentrations of estradiol-17 beta in fluid of ovarian cysts were significantly lower than those of large follicles. The number of luteinizing hormone (LH) receptors in non-luteinized cysts (plasma concentration of progesterone <1 ng/ml) was significantly fewer than that of luteinized cysts (plasma concentration of progesterone *1 ng/ml). More estradiol-17 beta in fluid of non-luteinized cysts was observed when they possessed relatively many LH receptors.3. The addition of cortisol to granulosa cells in vitro caused a significant decrease in the unmber of LH receptors and the estradiol-17 beta production.4. The addition of progesterone to the median eminence o … More f the hypothalamus in in vitro perfusion experiments caused a significant decrease in the GnRH release.5. Ovarian cysts were experimentally induced by the treatment with adrenocorticotropic hormone (ACTH). During development of ACTH-induced cysts, LH and follicle stimulating hormone (FSH) surges from the pituitary which normally found prior to ovulation were disappeared. After ACTH treatment, the increase in plasma progesterone and cortisol levels accompanied by low estradiol- 17 beta level were observed, suggesting that these changes could be the reason for the disappearance of LH and FSH surges.6. Studies on the treatment of ovarian cysts with human chorionic gonadotrpin (hCG) revealed that the efficacy of the drug could be estimated to some extent by determining the concentrations of progesterone in peripheral plasma and estradiolー17 beta in fluid of ovarian cysts.These results suggest that, as a possible mechanism of development of ovarian cysts, cortisol and progesterone production from adrenal are increased by ACTH stimulation under continuous stress, and the former interferes with follicular maturation by decreasing the number of LH receptors and the estradiol-17 beta production in granulosa cell, while the latter inhibits directly GnRH release from the median eminence, thereby resulting in the disappearance of gonadotropin surge and ovulation failure. They also indicate that there is a constant relation between the efficacy of treatment and endocrinological status in cows with ovarian cysts. Less

本文对牛卵巢囊肿的发育内分泌学和治疗进行了研究，得到以下结果：卵巢囊肿奶牛垂体前叶促性腺激素释放激素（GnRH）受体的数量和亲和性未见明显异常。卵巢囊肿液中促性腺激素受体数量和雌二醇-17 β浓度明显低于大卵泡。血浆黄体酮浓度<1 ng/ml的非黄体化囊肿中黄体生成素（LH）受体数量明显少于血浆黄体化囊肿（血浆黄体酮浓度*1 ng/ml）。非黄体化囊肿具有较多的黄体生成素受体时，其液中雌二醇-17 β含量较高。体外颗粒细胞中添加皮质醇导致LH受体数量和雌二醇-17 β的产生显著减少。在体外灌注实验中，下丘脑正中隆起处添加孕酮可显著降低GnRH的释放。实验采用促肾上腺皮质激素（ACTH）诱导卵巢囊肿。在acth诱导囊肿的发展过程中，通常在排卵前发现的垂体LH和促卵泡激素（FSH）激增消失了。促肾上腺皮质激素治疗后，血浆中黄体酮和皮质醇水平升高，同时雌二醇- 17 β水平降低，提示这些变化可能是LH和FSH升高消失的原因。人绒毛膜促性腺激素（hCG）治疗卵巢囊肿的研究表明，通过测定外周血浆中黄体酮和卵巢囊肿液中雌二醇- 17 β的浓度，可以在一定程度上估计药物的疗效。上述结果提示，持续应激下ACTH刺激肾上腺皮质激素分泌皮质醇和黄体酮增加可能是卵巢囊肿发生的机制之一，前者通过减少颗粒细胞中LH受体数量和雌二醇-17 β的产生干扰卵泡成熟，后者直接抑制正中隆起GnRH的释放。从而导致促性腺激素激增消失和排卵失败。它们还表明，卵巢囊肿奶牛的治疗效果与内分泌状态之间存在恒定的关系。少

项目成果

森純一・稲葉俊夫・川手憲俊: "ウシ卵胞のゴナドトロピンレセプタ-濃度とステロイドホルモン濃度について" 家畜繁殖学雑誌. 35. 106-112 (1989)

Junichi Mori、Toshio Inaba、Noritoshi Kawate：“牛卵泡中促性腺激素受体浓度和类固醇激素浓度”《牲畜育种科学杂志》35. 106-112 (1989)。

MORI J.,INABA T.,KAWATE N.: "GonadotropinーReleasing Hormone Receptors in Anterior Pituitaries of Cattle during Estrous Cycle" Animal Reproduction Science. 24. 185-191 (1991)

MORI J.、Inaba T.、KAWATE N.：“发情周期牛垂体前叶的促性腺激素释放激素受体”动物生殖科学 24. 185-191 (1991)。

Junichi Mori, Toshio Inaba, Noritoshi Kawate: "GonadotropinーReleasing Hormone Receptors in Anterior Pituitaries of Cattle during Estrous Cycle" Animal Reproduction Science. 24. 185-191 (1991)

Junichi Mori、Toshio Inaba、Noritoshi Kawate：“发情周期牛垂体前叶的促性腺激素释放激素受体”动物生殖科学 24. 185-191 (1991)。

MORI J.,INABA T.,KAWATE N.: "A Quantitative Comparison in the Bovine of Steroids and Gonadotropin Receptors in Normally Developing Follicles and in Follicular and Luteinized Cysts" Animal Reproduction Science. 23. 273-281 (1990)

MORI J.、INABA T.、KAWATE N.：“牛类固醇和促性腺激素受体在正常发育的卵泡以及卵泡和黄素化囊肿中的定量比较”动物生殖科学。

Junichi Mori, Toshio Inaba, Noritoshi Kawate: "Numbers of Gonadotropin Receptors and Concentrations of steroid Hormones in Bovine Follicles" The Japanese Journal of Animal Reproduction. 35. 106-112 (1989)

Junichi Mori、Toshio Inaba、Noritoshi Kawate：“牛卵泡中促性腺激素受体的数量和类固醇激素的浓度”日本动物生殖杂志。