Physiological and Morphological Studies on Characteristics of Nociceptive Primary Afferent Neurons (Polymodal Receptors).

伤害性初级传入神经元（多模式受体）特征的生理学和形态学研究。

• 批准号: 01480122
• 负责人: KUMAZAWA Takao
• 金额: $ 3.84万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480122/
• 关键词: Pain; Nociceptor; Inflammation; Intraspinal termination; Neurogenic inflammation; Neuropeptide; Inflammatory mediator; 脊髄内終末; 脊髄内終未

1. In order to clarify changes in polymodal receptor activities in inflammation, effects of inflammatory mediators on the heat response of testicular polymodal receptors were studied in vitro using testis-spermatic nerve preparations. ProstaglandinE_2 augmented the heat responses from the concentration 100 times higher than that needed to augment the Bradykinin (BK) response. 5-HT augmented both responses in the same range of concentration. Histamine (His) had different effects : His suppressed or augmented the BK response depending on whether His induced substantial increase in discharge rate or not, whereas it always augmented the heat response. BK augmented the heat response. None of these mediators could augment the heat response so strong as 55^ﾟC-30s heat stimulation did. BK response was revealed to be mediated by B2 type receptor. 2. Peripheral structure of identified testicular polymodal receptors was clarified by EM. Spinal termination of identified testicular polymodal receptors was traced mainly in lamina I by intracellular injection of PHA-L. Double labeling with fluorescent dyes revealed that more than 90% DRG neurons innervating the testis contained CGRP. 3. Nervous component in changes in intraocular pressure induced by inflammatory mediators was clarified, but the possibility for substance P to be its neuronal mediator was not supported.

1.为了阐明炎症过程中多模式受体活性的变化，采用睾丸-精索神经标本，体外研究了炎症介质对睾丸多模式受体热反应的影响。前列腺素E_2增强热反应的浓度是增强缓激肽(BK)反应所需浓度的100倍。在相同的浓度范围内，5-羟色胺对上述两种反应均有增强作用。组胺(His)有不同的作用：His抑制或增强BK反应，取决于His是否引起放电频率的显著增加，而它总是增强热反应。BK增强了热反应。这些介质都不能像55ﾟC-30s热刺激那样增强热反应。BK反应可能由B2型受体介导。2.用电子显微镜明确了已鉴定的睾丸多通道受体的外周结构。通过细胞内注射PHA-L，已识别的睾丸多模式受体的脊髓终末主要在I层被追踪。荧光双标结果显示，支配睾丸的背根节神经元90%以上含有CGRP。3.阐明了炎症介质引起的眼压变化中的神经成分，但不支持P物质作为其神经介质的可能性。

项目成果

Mizumura, K. et al.,: "Comparison of effects of prostaglandinE_2 and I_2 on testicular nociceptor activities studied in vitro" Naunyn-Schmiedeberg's Arch. Pharmacol.,.

Mizumura, K. 等人：“体外研究的前列腺素 E_2 和 I_2 对睾丸伤害感受器活性的影响比较”Naunyn-Schmiedebergs Arch。

Mizumura,K.et al.: "Comparison of effects of prostaglandinE_2 and I_2 on testicular nociceptor activities studied in vitro" NaunynーSchmiedeberg's Arch.Pharmacol.

Mizumura, K. 等人：“体外研究的前列腺素 E_2 和 I_2 对睾丸伤害感受器活性的影响比较”Naunyn-Schmiedeberg 的 Arch.Pharmacol。

K.Mizumura: "Reversible suppressive effects of amfenac sodium on the response to bradykinin of the visceral polymodal receptor." Environmental Medicine. 33. 43-46 (1989)

K.Mizumura：“氨芬酸钠对内脏多模式受体缓激肽反应的可逆抑制作用。”

K.Yokoyama: "Implication of polymodal receptor activities in the intraocular pressure increase resulted from neurogenic inflammation." Japanese Journal of Ophthalmology. 34. (1990)

K.Yokoyama：“多模式受体活性对神经源性炎症引起的眼内压升高的影响。”

Kumazawa,T.: "Functions of the nociceptive primary neurons" Jpn.J.Physiol.40. 1-14 (1990)

Kumazawa,T.：“伤害性初级神经元的功能”Jpn.J.Physiol.40。