Phenotypic Plasticity of Grafted Catecholaminergic Neurons and its Regulatory Mechanisms.
嫁接儿茶酚胺能神经元的表型可塑性及其调节机制。
基本信息
- 批准号:01480127
- 负责人:
- 金额:$ 4.42万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1989
- 资助国家:日本
- 起止时间:1989 至 1990
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. Using model rats of hemi-parkinson's disease, the mechanisms of functional recovery after grafting of dopaminergic cells were investigated. We found : 1) the survival of TH mRNA/TH positive cells, 2) increase of dopamine synthesis/release, 3) synaptic connections, 4) recovery of dopamine transporter and 5) normalization of D_1 and D_2 receptor-bindings.2. Bovine total ganglioside (tGS), sialosyl cholesterol (ganglioside analogue), IL-6 (B cell stimulating cytokine) facilitated the development/growth of grafted dopaminergic cells, and promoted the motor recovery.3.1) grafted chromaffin (adrenergic) cells transmute to dopaminergic neurons in dopamine-depleted striatum, 2) grafted noradrenergic cells transmute to dopaminergic cells in dopa-mine-depleted striatum but not in the lateral ventricle, 3) dopaminergic neurons grow much better in dopamine-depleted striatum than in normal striatum or the cortex, 4) grafted cholinergic neurons grow in a similar fashion in normal and dopamine-depleted striatum, 5) co-cultured midbrain neurons (partly dopaminergic neurons) extend more neurites faster toward the dopamine-depleted than toward the intact striatal tissue. These evidences suggest the increase in synthesis/release of some trophic factors for dopaminergic neurons in dopamine-depleted striatum.4. In soluble fraction of dopamine-depleted striatal tissue a few acidic proteins increased. The purification/identification of these proteins is under way.
1. 采用半帕金森病模型大鼠,研究多巴胺能细胞移植后功能恢复的机制。我们发现:1)TH mRNA/TH阳性细胞存活,2)多巴胺合成/释放增加,3)突触连接,4)多巴胺转运体恢复,5)D_1和D_2受体结合正常化。牛总神经节苷脂(tGS)、唾液酰胆固醇(神经节苷类似物)、IL-6 (B细胞刺激细胞因子)促进了移植多巴胺能细胞的发育/生长,促进了运动恢复。3.1)移植的染色质(肾上腺素能)细胞在多巴胺缺失纹状体中转化为多巴胺能神经元,2)移植的去甲肾上腺素能细胞在多巴胺缺失纹状体中转化为多巴胺能细胞,而在侧脑室中不转化为多巴胺能细胞。3)多巴胺能神经元在多巴胺缺失纹状体中比在正常纹状体或皮质中生长得更好;4)移植的胆碱能神经元在正常纹状体和多巴胺缺失纹状体中生长的方式相似;5)共培养的中脑神经元(部分多巴胺能神经元)向多巴胺缺失纹状体延伸的神经突比向完整纹状体组织延伸的神经突更快。这些证据表明多巴胺缺失纹状体中多巴胺能神经元的某些营养因子的合成/释放增加。在多巴胺缺失纹状体组织的可溶性部分中,一些酸性蛋白增加。这些蛋白的纯化/鉴定正在进行中。
项目成果
期刊论文数量(32)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Phenotypic plasticity of locus coeruleus noradrenergic neurons after transplantation into the dopamineーdepleted caudate im the rat." Prog.Brain Res.82. 515-521 (1990)
Nishino, H.、Hashitani, T.、Kumazaki, M. 等人:“移植到大鼠多巴胺耗尽的尾状核后蓝斑去甲肾上腺素能神经元的表型可塑性。Prog.Brain Res.82。” 1990)
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- 影响因子:0
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Nishino,H.,Hashitani,T.,Isobe Y.et al.: "tGS ganglioside induces peculiar morphological features in grafted dopaminergic cells and promotes motor recovery in rats with unilateral lesions in the nigrostriatal dopamine pathway." Brain Res.534. 73-82 (1990)
Nishino,H.,Hashitani,T.,Isobe Y.等人:“tGS 神经节苷脂在移植的多巴胺能细胞中诱导特殊的形态特征,并促进黑质纹状体多巴胺途径单侧损伤的大鼠的运动恢复。”
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- 影响因子:0
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Nishino,H.,Hashitani,T.and Kumazaki,M.: "Phenotypic plasticity of grafted catecholaminergic cells in the dopamineーdepleted caudate nucleus in the rats." Neurosci.Res.,Suppl.13. S54-60 (1989)
Nishino, H.、Hashitani, T. 和 Kumazaki, M.:“大鼠多巴胺耗尽尾状核中移植的儿茶酚胺能细胞的表型可塑性。Neurosci.Res.,Suppl.13(1989)。”
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Ishida,Y.,Hashitani,T.,Kumazaki,M.et al.: "Behavioral and biochemical effects of intraーaccumbens dopaminergic grafts." Brain Res.Bull.24. 487-492 (1990)
Ishida, Y.、Hashitani, T.、Kumazaki, M. 等人:“伏隔内多巴胺能移植物的行为和生化效应。”Brain Res.Bull.24 (1990)。
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- 影响因子:0
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Nishino,H.,Hashitani,T.,Kumazaki,M.et al.: "Grafting of catecholaminergic cells in the mammalian brain and reconstruction of disturbed function:basic problems to be solved." Comp.Biochem.Physiol.(1990)
Nishino,H.,Hashitani,T.,Kumazaki,M.等人:“在哺乳动物大脑中移植儿茶酚胺能细胞并重建受干扰的功能:需要解决的基本问题。”
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NISHINO Hitoo其他文献
NISHINO Hitoo的其他文献
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{{ truncateString('NISHINO Hitoo', 18)}}的其他基金
Development of stem (neural stem-, embryonic stem-, medullary stem) cells to dopaminergic neurons and their application to reconstruction medicine.
干细胞(神经干细胞、胚胎干细胞、髓质干细胞)发育为多巴胺能神经元及其在重建医学中的应用。
- 批准号:
15200026 - 财政年份:2003
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Striatal vulnerability and functional repair by neural grafts
神经移植物的纹状体脆弱性和功能修复
- 批准号:
10044311 - 财政年份:1998
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Development of Huntington's model animals and protection of the symptom by antisense strategy
亨廷顿舞蹈症模型动物的开发和反义策略对症状的保护
- 批准号:
09558104 - 财政年份:1997
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Mechanisms of cell in following brain ischemia and reconstruction of disturbed function by neural graft
脑缺血后的细胞机制及神经移植重建受损功能
- 批准号:
07458223 - 财政年份:1995
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of catecholamine producing cells by the transfection of CV40 TS gene and its application to intracranial neural transplantation.
CV40 TS基因转染儿茶酚胺产生细胞的研制及其在颅内神经移植中的应用
- 批准号:
04557005 - 财政年份:1992
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Identification of dopaminergic neuron trophic factor using subtraction hybridization and bioassay
使用消减杂交和生物测定法鉴定多巴胺能神经元营养因子
- 批准号:
03454133 - 财政年份:1991
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
The mechanism of differentiation of transplanted chromaffin cells into dopaminergic neurons.
移植的嗜铬细胞分化为多巴胺能神经元的机制。
- 批准号:
62570054 - 财政年份:1987
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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