Isolation of cDNA Clone Encodes Monoclonal Nonspecific Suppressor Factor (MNSF)

编码单克隆非特异性抑制因子 (MNSF) 的 cDNA 克隆的分离

• 批准号: 01480219
• 负责人: TSUNEMATSU Tokugoro
• 金额: $ 4.03万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480219/
• 关键词: monoclonal; nonspecific; suppressor factor; hybridoma; ハイブリド-マ

We obtained MNSF from supernatant of murine T cell hybridoma. MNSF was isolated with the use of anti-MNSF monoclonal antibody, following which it was further purified by reverse-phase high pressure liquid chromatography (HPLC). MNSF was analyzed by auto amino acid sequence analyzer. The N-terminal amino acid sequence data showed no homology to any known biologically active proteins including nonspecific suppressor factor.Unfortunately, the sequence data include several ambiguous residues. Self-aggregation of MNSF also makes it difficult to isolate the cDNA. Further studies are under way to prepare MNSF gene with the use of polymelase chain reaction (PCR).

我们从小鼠T细胞杂交瘤上清中获得MNSF。利用抗MNSF单克隆抗体分离MNSF，然后通过反相高压液相色谱（HPLC）进一步纯化。用全自动氨基酸序列分析仪分析MNSF。N-末端氨基酸序列数据显示与任何已知的生物活性蛋白包括非特异性抑制因子没有同源性，不幸的是，序列数据包括几个模糊的残基。MNSF的自聚集也使得难以分离cDNA。利用聚合酶链反应（PCR）制备MNSF基因的研究正在进行中。

项目成果

Morihiko Nakamura: "Interferon r (IFNーr) enhances the expression of cell surface receptor for monoclonal nonspecific suppressor factor (MNSF)"

Morihiko Nakamura：“干扰素 r (IFN-r) 增强单克隆非特异性抑制因子 (MNSF) 细胞表面受体的表达”

Morihiko Nakamura et al.: "Characterization of N-terminal amino acid sequences of monoclonal nonspecific suppressor factor (MNSF)"

Morihiko Nakamura 等人：“单克隆非特异性抑制因子 (MNSF) N 末端氨基酸序列的表征”

Morihiko Nakamura: "Charcterization of cellーsurface receptors for monoclonal nonspectific suppressor factor (MNSF)" Cellular Immunology. 130. 281-290 (1990)

Morihiko Nakamura：“单克隆非特异性抑制因子 (MNSF) 细胞表面受体的表征”《细胞免疫学》130. 281-290 (1990)。

Morihiko Nakamura: "Characterization of cell surface receptors for monoclonal nonspecific suppressor factor(MNSF)" Cellular Immunology.

Morihiko Nakamura：“单克隆非特异性抑制因子 (MNSF) 细胞表面受体的表征”细胞免疫学。

Morihiko Nakamura: "Characterization of Nーterminal amino acid sequences of monoclonal nonspecific suppressor factor (MNSF)"

Morihiko Nakamura：“单克隆非特异性抑制因子 (MNSF) N 端氨基酸序列的表征”