Research on Expression of Myelin-Associated Glycoprotein in Regeneration of Central Nervous System

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The myelin-associated glycoprotein (MAG) is minor myelin membrans proteins and may play a role in cell to cell interaction as a member of immunoglobulin superfamily. We completed cDNA cloning of mouse MAG and found two kinds of MAG isoforms (small-MAG : S-MAG, large-MAG : L-MAG) which are expressed by alter native splicing mechanism. To clarify one of mechanisms of regeneration in nervous system, we examined roles of MAG on remyelination after damage of nervous system. The results of this research program are as follows.1) We established an experimental model of demyelination and remyelination by Cuprizon intoxication.2) An model of secondary demyelination by occlusion of middle cerebral artery was established.3) We prepared an anti-L-MAG and an anti-S-MAG antibodies from synthetic peptides of c-termini to L-MAG and S-MAG respectively.4) The L-MAG isoform was induced during remyelinating stage after demyelination by Cuprison intoxication.5) Changes of brain proteins including MAG were examined.6) In the quaking mutant mouse, L-MAG was scarcely expressed throughout development.Therefore, L-MAG is revealed to be important for normal myelination.7) We have completed cDNA cloning of human MAG and obtained only L-MAG clone.