Endogenous

内源性

• 批准号: 01480247
• 负责人: OGIHARA Toshio
• 金额: $ 4.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480247/
• 关键词: Essential hypertension; Natriuretic factor; Anti-ouabain antibody; Prostaglandin derivative; Na^+, K^+-ATPase; 内因性ジキタリス様物質; ウアバイン様免疫活性; 抗ウアドインモノクロナル抗体; 内因性ジギタリス様物質; ウワバイン様免疫活性; 抗ウワバインモノクロナル抗体; 立体特異性

Endogenous digitalis-like factor (EDLF) is thought to be one of the causal factor for the development of essential hypertension although the precise nature of the factor is not clear. In the present study, we investigated about the participation of circulating EDLF in the development of hypertension, and partially purified the EDLF. Circulating level of the EDLF was measured by radioimmuno assay using anti-oubain monoclonal antibody. The plasma level of EDLF was increased in patients with essential hypertension compared to that in normal subjects. The level was significantly decreased by sodium depletion, and significantly increased with high salt diet in patients with essential hypertension, and was significantly decreased after adrenectomy in a patient with primary aldosteronism. 1 The EDLF was partially purified from, plasma from patients with essential hypertension. Lipid fraction extracted from the plasma by methanol/chloroform was applied to high-performance liquid chromatogrhy with CLC-CN, column and HCG-15SH column. The molecular weight of the partially, purified EDLF. was about 600. We also developed a monoclonal antibody to oubain, which recognized lacton ring and trans-cis structuring of the BCD rings.

内源性洋地黄样因子（EDLF）被认为是原发性高血压发生发展的原因之一，尽管该因子的确切性质尚不清楚。本研究探讨了循环中EDLF在高血压发生发展中的作用，并对EDLF进行了部分纯化。采用放射免疫法测定EDLF水平。原发性高血压患者血浆EDLF水平明显高于正常人。钠耗竭后该水平显著降低，原发性高血压患者高盐饮食后该水平显著升高，原发性醛固酮增多症患者肾上腺切除术后该水平显著降低。1从原发性高血压患者血浆中部分纯化EDLF。用甲醇/氯仿提取血浆中的脂质组分，用高效液相色谱法，以CLC-CN柱和HCG-15 SH柱进行分离。部分纯化的EDLF的分子量。大约是600。我们还开发了一种单克隆抗体，识别内酯环和BCD环的反式-顺式结构。

项目成果

Ogihara Toshio,et al.: "Changes in plasma renin activity and aldosterone concentration in response to endothelin injection in dogs." Acta Endocrinologica. 121. 5685-5689 (1989)

Ogihara Toshio 等人：“狗内皮素注射后血浆肾素活性和醛固酮浓度的变化。”

荻原 俊男ら: "ウアバイン様ナトリウム利尿因子" 日本臨床代謝学会記録. 27. 18-19 (1991)

Toshio Ogihara 等：“Ouabain 样利尿钠因子”日本临床代谢学会记录 27. 18-19 (1991)。

Ogihara T, Morimoto S and Tabuchi Y.: "The role of endothelin in blood pressure regulation" In Peptide Regulation of Cardiovascular Function. Ed. by Imura H., Academic Press Inc.259-272 (1991)

Ogihara T、Morimoto S 和 Tabuchi Y.：“内皮素在血压调节中的作用”，心血管功能的肽调节。

Ogihara Toshio,et al.: "Effect of ouabain on hemodynamics in acute volume expanded hypertensive dogs." Basic Research in Cardiology. 84. 319-325 (1989)

Ogihara Toshio 等人：“哇巴因对急性容量扩张高血压犬血流动力学的影响。”

Ogihara T, Morita R, Morimoto S, Imanaka S and Fukuo K.: "Intracellular signal transduction evoked by low-density lipoprotein in vascular smooth muscle cells." In Calcium-regulating Hormones. I. Role in Disease and Aging. 90. 116-121 (1991)

Ogihara T、Morita R、Morimoto S、Imanaka S 和 Fukuo K.：“血管平滑肌细胞中低密度脂蛋白引起的细胞内信号转导。”