Endogenous

内源性地黄样因子（Endogenous digitalis-like factor， EDLF）被认为是原发性高血压发生的原因之一，但其确切性质尚不清楚。在本研究中，我们探讨了循环EDLF参与高血压的发展，并部分纯化了EDLF。用抗麻豆素单克隆抗体放射免疫法测定EDLF的循环水平。与正常人相比，原发性高血压患者血浆EDLF水平升高。在原发性高血压患者中，高盐饮食显著升高，在原发性醛固酮增多症患者中，肾上腺切除术后显著降低。EDLF从原发性高血压患者的血浆中部分纯化。采用甲醇/氯仿法提取血浆脂质组分，采用hplc - cn柱和HCG-15SH柱进行高效液相色谱。部分纯化的EDLF的分子量。大约是600。我们还开发了一种单克隆抗体，可以识别乳酸菌环和BCD环的反式结构。

Endogenous digitalis-like factor (EDLF) is thought to be one of the causal factor for the development of essential hypertension although the precise nature of the factor is not clear. In the present study, we investigated about the participation of circulating EDLF in the development of hypertension, and partially purified the EDLF. Circulating level of the EDLF was measured by radioimmuno assay using anti-oubain monoclonal antibody. The plasma level of EDLF was increased in patients with essential hypertension compared to that in normal subjects. The level was significantly decreased by sodium depletion, and significantly increased with high salt diet in patients with essential hypertension, and was significantly decreased after adrenectomy in a patient with primary aldosteronism. 1 The EDLF was partially purified from, plasma from patients with essential hypertension. Lipid fraction extracted from the plasma by methanol/chloroform was applied to high-performance liquid chromatogrhy with CLC-CN, column and HCG-15SH column. The molecular weight of the partially, purified EDLF. was about 600. We also developed a monoclonal antibody to oubain, which recognized lacton ring and trans-cis structuring of the BCD rings.