Studies on the abnormality in human proTRH cDNA of patients with TRH deficiency.

TRH缺乏症患者人proTRH cDNA异常的研究。

• 批准号: 01480284
• 负责人: MORI Masafumi
• 金额: $ 4.29万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480284/
• 关键词: proTRH; proTRH cDNA; mRNA; PCR; Hypothalamus; TRH deficiency; Depression; proTRHcDNA; Hypothelamas; TRHdeficienay; Depressou; TRH; cDNA; 視床下部

RNAs derived from human and mouse hypothalami were reversetranscrived to each cDNA. Using each cDNA as a temprate and primers corresponding to the sequence of cDNA encoding rat proTRH, polymerase chain reaction was performed. The resulted cDNA was subcloned into pGEM3Z vector and the sequence determined. The human proTRH cDNA was transfected into CHO cells and resulted proteins were analyzed for proTRH using the specific anti-proTRH antibody. Restriction fragment length polymorphism using the human proTRH cDNA showed no significant difference in DNAs of normal control subjects and patients with TRH-deficiency and dpression.

将来自人类和小鼠下丘脑的rna逆转录到每个cDNA上。以每条cDNA为温剂，与编码大鼠promh的cDNA序列对应引物，进行聚合酶链反应。将得到的cDNA亚克隆到pGEM3Z载体上，并确定其序列。将人prohncdna转染CHO细胞，用特异性抗prohn抗体分析其蛋白。利用人prodhcdna进行限制性内切片段长度多态性分析，正常对照者与trh缺乏症和抑郁症患者的dna无显著差异。

项目成果

Yamada M,et al: "Influence of hypothyrordtion on TRH Couecnprecoeis and propro TRH in RNA lerels in rat hypothalauns:a sinple and reliable lueithod to detect prepro TRH in RNA level" Neuroeudecriuolagy. 55. 317-320 (1992)

Yamada M,et al：“甲状腺功能减退对大鼠下丘脑 RNA 水平中 TRH Couecnprecoeis 和 propro TRH 的影响：一种简单且可靠的方法来检测 RNA 水平中的 prepro TRH”Neuroeudecriuolagy。

Satoh T.et al: "Cloniug of the mouse hypothacamies preprothyrotiopinreleassug homdone(TRH)cDNA and tissite disthibucou of its mRNA" Mol Brain Rev. (1992)

Satoh T.等人：“小鼠下丘脑前原甲状腺素释放激素 (TRH)cDNA 的克隆及其 mRNA 的 tissite disthibucou” Mol Brain Rev. (1992)

Mori M.etal: "Stimulation by a TRH precursor,TRHーGly,of TSH and PRL secretion in rats:effect of staruation" Neuropeptides. 16. 57-62 (1990)

Mori M.等人：“TRH 前体 TRH-Gly 对大鼠 TSH 和 PRL 分泌的刺激：启动效应”Neuropeptides 16. 57-62 (1990)。

Satoh T.etal: "Clonicy of the mouse gypothalances preprothyrotropin-releaion houmone (TRH) cDNA and tissue distrivution of its mRNA" Mol Brain Rev. (1992)

Satoh T.etal：“小鼠 gypothalances 前促甲状腺素释放激素 (TRH) cDNA 的克隆性及其 mRNA 的组织分布”Mol Brain Rev. (1992)

Mori M et al: "Different postーtrauslational processin of human Preprothyro tropinーvelasry human in the human placeuta and hypo thalowus." J elin Eudocrinol Metab. (1992)

Mori M 等人：“人前甲状腺素-velasry 人在胎盘和下丘脑中的不同的插入后加工过程”（J elin Eudocrinol Metab）。