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Studies on the abnormality in human proTRH cDNA of patients with TRH deficiency.

Studies on the abnormality in human proTRH cDNA of patients with TRH deficiency.
TRH缺乏症患者人proTRH cDNA异常的研究。
批准号:
01480284
负责人:
MORI Masafumi
金额:
$4.29万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1989
资助国家:
日本
项目状态:
已结题
起止时间:
1989 至 1991

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中文摘要
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英文摘要
RNAs derived from human and mouse hypothalami were reversetranscrived to each cDNA. Using each cDNA as a temprate and primers corresponding to the sequence of cDNA encoding rat proTRH, polymerase chain reaction was performed. The resulted cDNA was subcloned into pGEM3Z vector and the sequence determined. The human proTRH cDNA was transfected into CHO cells and resulted proteins were analyzed for proTRH using the specific anti-proTRH antibody. Restriction fragment length polymorphism using the human proTRH cDNA showed no significant difference in DNAs of normal control subjects and patients with TRH-deficiency and dpression.
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Yamada M,et al: "Influence of hypothyrordtion on TRH Couecnprecoeis and propro TRH in RNA lerels in rat hypothalauns:a sinple and reliable lueithod to detect prepro TRH in RNA level" Neuroeudecriuolagy. 55. 317-320 (1992)
Yamada M,et al:“甲状腺功能减退对大鼠下丘脑 RNA 水平中 TRH Couecnprecoeis 和 propro TRH 的影响:一种简单且可靠的方法来检测 RNA 水平中的 prepro TRH”Neuroeudecriuolagy。
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Satoh T.et al: "Cloniug of the mouse hypothacamies preprothyrotiopinreleassug homdone(TRH)cDNA and tissite disthibucou of its mRNA" Mol Brain Rev. (1992)
Satoh T.等人:“小鼠下丘脑前原甲状腺素释放激素 (TRH)cDNA 的克隆及其 mRNA 的 tissite disthibucou” Mol Brain Rev. (1992)
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Mori M.etal: "Stimulation by a TRH precursor,TRHーGly,of TSH and PRL secretion in rats:effect of staruation" Neuropeptides. 16. 57-62 (1990)
Mori M.等人:“TRH 前体 TRH-Gly 对大鼠 TSH 和 PRL 分泌的刺激:启动效应”Neuropeptides 16. 57-62 (1990)。
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Satoh T.etal: "Clonicy of the mouse gypothalances preprothyrotropin-releaion houmone (TRH) cDNA and tissue distrivution of its mRNA" Mol Brain Rev. (1992)
Satoh T.etal:“小鼠 gypothalances 前促甲状腺素释放激素 (TRH) cDNA 的克隆性及其 mRNA 的组织分布”Mol Brain Rev. (1992)
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23
    System Construction for Research Information Triage and Investigation Action Strategy under Widespread and Large Scale Earthquake Disaster
    • 批准号:
      21560586
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.0万
    • 财政年份:
      2009
    • 负责人:
      MORI Masafumi
    • 依托单位: