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Creation and analysis of the animal models with chronic compression myelopathy.

慢性压迫性脊髓病动物模型的创建和分析。

基本信息

批准号：
01480366
负责人：
MIMATSU Kentaro
金额：
$ 4.48万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

To determine the functional changes occurring in the early period after the development of chronic cord compression, we created an ossification of the yellow ligament(OYL)model, which is a model of chronic cord compression, in domestic rabbits using bone morphogenetic protein(BHP), and monitored evoked spinal cord potentials(ESCP). Sixty-seven rabbits were used for these experiments. Myelography, ESCP measurements, and histological examinations were performed in 16 of the OYL and 6 of the healthy control rabbits.At an average of 40.6 weeks after creitio, of the OYL model, a mean cord compression ratio of 15.3% was found. When the sciatic nerve was stimulated, no significant differences were found in the latency of the ESCP first wave between the OYL and control rabbits, whereas with stimulation delivered to the motor area the first wave latency differed significantly(P<0.005)between the 2 groups when the cord compression ratio in the OYL rabbits was *10%. Histological changes consisting primarily of a reduction in the number of large fibers in the dorsal column were found in 3 of the 4 OYL rabbits showing a cord compression ratio of *20%.Our results demonstrate that(1)mild degrees of cord compression produce greater ESCP alterations in chronic than in acute cord compression, (2)chronic cord compression from the dorsal side produces injury in the superficial layer of the dorsal column and dorso-lateral column, and(3)changes in ESCP precede the development of paralysis and histological changes.

为了确定慢性脊髓压迫发展后早期发生的功能变化，我们使用骨形态发生蛋白（BHP）在家兔身上建立了慢性脊髓压迫模型黄韧带骨化（OYL）模型，并监测了脊髓诱发电位（ESCP）。实验用了67只兔子。对16只OYL兔和6只健康对照兔进行了脊髓造影、ESCP测量和组织学检查。在出生后平均40.6周，OYL模型的平均脐带压缩率为15.3%。当刺激坐骨神经时，OYL兔与对照组的ESCP第一波潜伏期无显著差异，而当脊髓压迫比为*10%时，两组间的ESCP第一波潜伏期差异显著（P<0.005）。4只OYL兔中有3只的组织学改变主要是脊背柱大纤维数量减少，脊髓压缩率为*20%。我们的研究结果表明：(1)轻度的慢性脊髓受压比急性脊髓受压产生更大的ESCP改变，(2)来自背侧的慢性脊髓受压产生背柱和背外侧柱浅层损伤，(3)ESCP的变化先于麻痹和组织学改变的发展。