A study on the development of fetal behavior and central nervous system

胎儿行为及中枢神经系统发育的研究

• 批准号: 01480389
• 负责人: KUWABARA Yoshinori
• 金额: $ 4.16万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480389/
• 关键词: artificial placent; ECMO; fetal goat; fetal behavior; eye movements; central vervous system; 胎児中枢神経系機能; 胎児生理; 睡眠覚醒; behavioral state; 子宮外胎仔保育; 子宮外胎仔保育法; 胎児中枢神経系

We developed a system of extrauterine incubation of a goat fetus, in which the fetus in surrounded by artificial amniotic fluid and connected to the circuit with an ECMO (extracorporeal membrane oxygenator) by the umbilical arteries and veins.The mean duration of the incubation was 83 hours and 48 minutes with a recent improvement. Tne longest one was 143 h.and 48 min.in this series. Slowly progress of circulatory depression and systemic body edema were thought to be the main cause of fetal death.EEG,EOG,intratracheal presuure, EMG of nucal and thigh muscle were monitored continuously. Four categories of behavioral states were defined on the basis of the findings of these monitoring and the fetal movements observed with naked eyes.The effects of PGE_2, and PGD_2, which are known as regulators of sleep-wakefulness cycle, on behavioral states defined above were investigated to clarify the meanings of these behavioral states. As a result of these experiments, it was suggested that four categories of behavioral states in extra-uterine fetal goats were deep Non-REM sleep, shallow Non-REM sleep, REM sleep and wakefulness.The detail is shown in the text of the report.

我们建立了一个山羊胎儿的宫外培养系统，胎儿被人工羊水包围，通过脐动、静脉与体外膜肺氧合器（ECMO）相连，平均培养时间为83小时48分钟。最长的一个是143小时和48个。min.in持续监测脑电图、眼电图、颅内压、膈肌肌电图、臀肌电图等指标，并与正常对照组比较。根据监测结果和肉眼观察的胎动，将行为状态分为四类，并探讨了睡眠-觉醒周期调节因子PGE_2和PGD_2对上述行为状态的影响，以阐明这些行为状态的含义。这些实验的结果表明，子宫外胚胎山羊的四种行为状态是深度非快速眼动睡眠、浅度非快速眼动睡眠、快速眼动睡眠和清醒。详细情况见报告正文。

项目成果

海野信也、桑原慶紀他: "人工子宮の開発:A-V ECMOによる子宮外胎仔保育における最適臍帯血流量に関する検討" 今日の人工臓器. 2(3). 197-204 (1992)

Shinya Unno、Yoshiki Kuwahara 等人：“人工子宫的发展：使用 A-V ECMO 进行宫外胎儿护理中最佳脐带血流的研究”《今日人工器官》197-204 (1992)。

海野 信也,桑原 慶紀 他: "〓帯動静脈AーV ECMOによる子宮外保育下ヤギ胎〓のガス交換動態" 人工臓器. 20. 1233-1238 (1991)

Shinya Unno、Yoshiki Kuwabara 等人：“使用脐带动静脉 AV ECMO 保存在子宫外的山羊胎儿的气体交换动力学”人工器官。

上妻 志郎: "胎児行動における中枢神経系制御機構の発達過程に関する総合的解析" 日本産科婦人科学会雑誌. 43. 834-842 (1991)

小祖马四郎：“胎儿行为中枢神经系统控制机制的发育过程的综合分析”日本妇产科学会杂志43。834-842（1991）。

海野 信也,桑原 慶紀,水野 正彦: "人工子宮開発の現階段" BIO INDUSTRY. 7. 322-331 (1990)

Shinya Unno、Yoshiki Kuwabara、Masahiko Mizuno：“人工子宫开发的当前步骤”BIO INDUSTRY。7. 322-331 (1990)。

海野信也、桑原慶紀他: "人工子宮の開発:A-V ECMOによる子宮外胎仔保育における酸素消費に関する検討" 胎児診断と治療. 5(3-4). 189-195 (1990)

Shinya Unno、Yoshiki Kuwahara 等人：“人工子宫的发展：使用 A-V ECMO 进行宫外胎儿护理期间的耗氧量研究”《胎儿诊断与治疗》5(3-4)。