Regulatory factors for differentiation and proliferation of small intestinal epithelium cells and adipose cells

动物通常由200多种高度分化的细胞组成。器官的形成和维持受膳食成分的影响。因此，评价影响细胞分化和/或增殖的外源性和/或内源性因素是非常重要的。本研究试图在模型培养细胞系统中检测这些因子，并对其结构、生理功能等进行分析，利用大鼠肠上皮细胞系IEC-16细胞，获得了抗肠卷曲富含抗原的单克隆抗体。本研究利用3 T3-L1（小鼠胚胎）和Ob 1771（遗传性肥胖小鼠脂肪组织）脂肪细胞系，发现了一种新的蛋白质生长因子（M.W.25，000），该因子能特异性地促进前脂肪细胞在各种哺乳动物脂肪组织中的增殖。脂肪组织中的这种生长因子响应能量摄入而发挥作用。这一因素可能为进一步阐明食物摄入与脂肪组织形成之间的关系提供有用的工具。

Animals usually are consisted of over 200 kinds of cells highly differentiate. The formation and maintenance of organ are affected by dietary components. So, it is very important that exogenous and/or endogenous factors, which affect on cell differentiation and/or proliferation, are evaluated. In this study, we tyied to detect these factors in model cultured cell systems and analyzed these structures, physiological functions and so on.By using rat intestinal cell line, IEC-16 cells, we obtained some monoclonal antibodies against intestinal cript-rich antigens. These monoclonal antibodies may be useful to evaluate the regulatory factors on intestinal epitherium cells.By using 3T3-L1 (mouse embryo) and Ob1771 (adipose tissue from genetically obesity mouse) adipose cell lines, we found a new protein growth factor (M.W.25,000) which specifically permits the proliferation of preadipocytes, on adipose tissue various mammalian containing human. This growth factor in adipose tissue functions in response to energy intake. This factor may provide a useful tool for further elucidation of the relationship between food intake and adipose tissue formation.