权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of the properties specific for sex hormone-dependent motoneurons

性激素依赖性运动神经元特异特性分析

基本信息

批准号：
02454122
负责人：
KUNO Motoi
金额：
$ 4.29万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454122/
关键词：
Motoneuron Hormone neuron death Trophic factor Electrophysiology

项目摘要

The spinal nucleus of the bulbocavernosus (SNB) in the rat innervates perineal striated muscles, including the bulbocavernosus, levator ani and external anal sphincter. These motoneurons are sexually dimorphic and responsive to the male hormone, testosterone. In female rats, the majority of SNB motoneurons die at early postnatal stages. In adult male rats, the size of SNB neurons is reduced by castration and increases by treatment with testosterone. The principal question posed in this research proposal is twofold : first, are the electrophysiological properties of the sex hormone-dependent SNB neurons are distinct from those of the motoneurons innervating skeletal muscles? ; second, is the effect of testosterone on neuronal size exerted directly on the SNB motoneurons or mediated by their target perineal muscles?Intracellular potentials and membrane currents were recorded from SNB motoneurons in male and female rats by the whole-cell configuration of patch-clamp techniques in thin sli … More ces of the lumbar spinal cord. The electrophysiological properties differed only quantitatively between the SNB motoneurons and the motoneurons innervating the hiridlimb muscles. It is concluded that the sex hormone-dependent SNB motoneurons and the motoneurons innervating the skeletal muscles cannot be classified into two distinct neuron types.The effects of castration and testosterone on neuronal size were compared between the SNB motoneurons reinnervating the original, hormone-sensitive perineal muscles and the SNB motoneurons reinnervating a skeletal muscle, the soleus muscle which lacks androgen sensitivity. The size of the SNB motoneurons reinnervating the original perineal muscles was reduced by castration and increased by testosterone manipulation. In contrast, the size of the SNB motoneurons reinnervating the grafted soleus muicle remained unchanged by castration or by treatment with testosterone. It is concluded that hormonal regulation of the size of SNB motoneurons is mediated by their target muscles. Less

大鼠球海绵体肌脊核（SNB）支配会阴横纹肌，包括球海绵体肌、提肛肌和肛门外括约肌。这些运动神经元是两性异形的，对雄性激素睾丸激素有反应。在雌性大鼠中，大多数SNB运动神经元在出生后早期死亡。在成年雄性大鼠中，SNB神经元的大小通过去势减少，并且通过睾酮治疗增加。在这项研究中提出的主要问题是双重的：第一，是性兴奋依赖SNB神经元的电生理特性是不同的运动神经元支配骨骼肌？第二，睾酮对神经元大小的影响是直接作用于SNB运动神经元还是通过其靶会阴肌介导的？用全细胞膜片钳技术记录了雄性和雌性大鼠SNB运动神经元的细胞内电位和膜电流。 ...更多信息腰脊髓的神经节SNB运动神经元和支配后肢肌的运动神经元之间的电生理特性仅在数量上存在差异。它的结论是，性激素依赖的SNB运动神经元和运动神经元支配的骨骼肌不能被分为两个不同的神经元type.The影响去势和睾酮对神经元的大小进行了比较SNB运动神经元再支配的原始，雄激素敏感的会阴肌肉和SNB运动神经元再支配的骨骼肌，比目鱼肌，缺乏雄激素敏感性。大小的SNB运动神经元重新支配原来的会阴肌肉减少去势和睾酮操作增加。与此相反，大小的SNB运动神经元再支配移植比目鱼肌muicle保持不变，去势或睾酮治疗。它的结论是SNB运动神经元的大小的激素调节介导的靶肌肉。少