Bone histomorphometric study of alveolar bone resorption and formation and root resorption on experimetal tooth movement of rats.

大鼠实验牙移动时牙槽骨吸收和形成及牙根吸收的骨组织形态计量学研究。

• 批准号: 02454470
• 负责人: TAKANO Teruko
• 金额: $ 4.54万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454470/
• 关键词: Experimental Tooth Movement; 1, 25 (OH)_2D_3; Local Application; osteoclast; Alveolar Bone Resorption and Formation; Root Resorption; Bone Histomorphometry

1. The purpose of the first study was to investigate the effect of local application of 1, 25 (OH)_2D_3 on osteoclast number induced by experimental tooth movement. A piece of orthodontic elastic band was inserted between the first and the second upper molars of Wistar male rats weighing 200 g. 20 mol of 1, 25 (OH)_2D_3 (10^<-12> -10^<-7> M)was injected locally in the submucosal palatal area of the root bifarcation of the right first molar. The left side was injected with phosphate-buffered sahne (PBS). The number of osteoclasts was counted in a 700x1050mum^2 area of the inter-radicular septum. The local injection ofl, 25 (OH)_2D_3 caused a dose-dependent increase in osteoclast number. The effect of 1, 25 (OH)_2D_3 appeared at 10^<-12>M and reached a response plateau at 10^<-10>M when greater than a 3-fold rise in osteocla number was attained compared with the PBS-treated controls. While the insertion of a pace of elastic band for three days induced a significant increase in the appear … More ance of osteoclasts in the alveolar bone, daily injections of 20 mul of 10^<-10>M 1, 25 (OH)_2D_3 for 3 days markedly stimulated the appearance of osteoclasts induced by inserting an elastic band. 1, 25 (OH)_2D_3 was synergistic with mechanical stimuli resulting in enhancement of the appearance of osteoclasts induced by mechanical stimuli alone. These findings suggested that the local application of 1, 25 (OH)_2D_3 acts directly to increase in osteoclast number and synergistically to stimulate osteoclastic bone resorption induced by mechanical stimuh.2. The purpose of the second study was to compare the amount and rate of tooth movement in young and mature rats administered 1, 25 (OH)_2D_3 simultaneous with application of mechanical force. In thirty 7-week-old and thirty 28-week-old male Wistar rats, the right maxillary first molar was moved buccally with a fixed appliance. The appliances delivered forces ranging from 5 to 20 g. Twenty ml of 1, 25 (OH)_2D_3 (10^<-10> and 10^<-8> mol/L)was injected locally in the submucosal palatal area of the root bifurcation of the right first molar. Ile left side was injected with phosphate-buffered saline (PBS). In young growing rats receiving 10-10 mol/L 1, 25 (OH)_2D_3 every three days, tooth movement significantly increased to 126% of that in PBS-injected control rats on day 20. In 1, 25 (OH)_2D_3-injected mature rats, tooth movement was stimulated markedly and increased with 10^<-10> mol/L to 245% and with 10^<-8> mol/L to 154% of the amount of tooth movement seen in the PBS-injected controls by the end of the experiment. PBS-injected rats had a plateau stage where tooth movement did not occur at all, while there was no such lag-time in the 1, 25 (OH)_2D_3-injected group which showed continuous tooth movement. The local injection of 1, 25 (OH)_2D_3 did not change serum calcium, phosphate and alkaline phosphatase activity and there were no apparent clinical or microscopic side-effects. Less

1.实验一探讨局部应用1，25(OH)_2D_3对实验性牙齿移动所致破骨细胞数量的影响。在体重200g的Wistar雄性大鼠第一、第二上磨牙之间插入正畸橡皮筋，在右侧第一磨牙根分叉粘膜下腭部局部注射20mol1，25(OH)_2D_3(10^&lt；-12；-7&gt；M)。左侧注射磷酸盐缓冲液(PBS)。对神经根间隔700×1050微米~2的面积进行破骨细胞计数。局部注射1，25(OH)_2D_3可引起破骨细胞数量增加，且呈剂量依赖性。1，25(OH)_2D_3的作用出现在10^~(-12)M，在10^~(-10)~(-10)M时达到反应平台期，此时破骨细胞数较PBS处理的对照组增加3倍以上。而插入速度为3天的橡皮筋引起的…显着增加牙槽骨内破骨细胞增多，每日注射20毫升10℃-10℃，连用3天，25(OH)_2D_3均能明显刺激松弛带诱导破骨细胞的出现。1，25(OH)2D3与机械刺激有协同作用，可促进单独机械刺激诱导的破骨细胞的出现。这些结果表明，局部应用1，25(OH)_2D_3直接增加破骨细胞数量，协同刺激机械刺激所致的破骨细胞性骨吸收。第二项研究的目的是比较1，25(OH)_2D_3同时施加机械力对幼年和成年大鼠牙齿移动量和移动速度的影响。30只7周龄和30只28周龄雄性Wistar大鼠，用固定矫治器将右侧上颌第一磨牙向颊移动。矫治器的受力范围为5~20g。在右侧第一磨牙根分叉的黏膜下腭部局部注射20毫升1，25(OH)_2D_3(10^&lt；-10；和10^&lt；-8；mol/L)。左侧注射磷酸盐缓冲液(PBS)。幼龄大鼠每3天注射10-10mol/L 1，25(OH)2D_3，第20天牙齿移动量显著增加至注射PBS对照组的126%；而注射1，25(OH)_2D_3的成年大鼠，牙齿移动受到明显刺激，实验结束时，10~(-10)mol/L和10~(-8)mol/L分别使牙齿移动量增加到对照组的245%和154%。注射PBS的大鼠出现牙齿完全不移动的平台期，而注射1，25(OH)2D3的大鼠不存在牙齿持续移动的滞后时间。局部注射1，25(OH)2D3对血清钙、磷和碱性磷酸酶无明显影响，无明显的临床和显微镜副作用。较少

项目成果

Takano-Yamamoto,T.,Soma,S.Nakagawa,K.,Kobayashi,Y.,Kawakami,M.and Sakuda,M.: "Comparison of the effects of hydro-static compressive force on glyco-saminoglycan synthesis and proliferation in rabbit chondrocytes from mandibulazr condylar cartilage,nasal se

Takano-Yamamoto，T.，Soma，S.Nakakawa，K.，Kobayashi，Y.，Kawakami，M.和Sakuda，M.：“静水压力对糖胺聚糖合成和增殖的影响的比较

T.TakanoーYamamoto,M.Kawakami and M.Sakuda: "Pulsing electromagnetic field enhance demineralized bone matrixinduced bone bony formation in a defect of premaxilla in rats." J.Dent.Res.

T.Takano-Yamamoto、M.Kawakami 和 M.Sakuda：“脉冲电磁场​​增强大鼠前上颌骨缺损中脱矿骨基质诱导的骨骨形成。”

TakanoーYamamoto,T.,Soma,S.,Nakagawa,K.,Kobayashi,Y.,Kawakami,M.and Sakuda,: "Comparison of the effets of hydrostatic compressive force on glycosaminoglycan synthesis and proliterafion in rabbit chondrocytes from mandibular condylar cartilage.nasal septum

Takano-Yamamoto, T.、Soma, S.、Nakakawa, K.、Kobayashi, Y.、Kawakami, M. 和 Sakuda，：“静水压力对兔下颌髁突软骨细胞糖胺聚糖合成和增殖的影响比较软骨鼻中隔

山本 照子: "エストロジェンと骨." 日骨代謝誌. 8(2). 1-14 (1990)

Teruko Yamamoto：“雌激素和骨骼。”日本骨代谢杂志 8(2)（1990）。

山本 照子: "メカニカルストレスと軟骨細胞ーウサギ頭蓋より分離した軟骨培積細胞に対する静水圧の影響ー" 日骨代謝誌. 8(2). 155-162 (1990)

Teruko Yamamoto：“机械应力和软骨细胞 - 静水压力对从兔颅骨中分离出的培养软骨细胞的影响”，日本骨代谢杂志 8(2) (1990)。