CHROMOSOME MAPPING OF MUTANT GENE CONCERNING GENETIC DISEASE IN MICE USING DNA POLYMORPHISMS

点击翻译按钮获取中文摘要

The lpr (lymphoproliferation) gene, which is an autosomal recessive gene causing autoimmune disease with features of human systemic lupus erythematosus and iventually death from severe immune-complex glomerulonephritis, has been mapped on mouse chromosome 19. To determine the exact chromosomal location of the lpr gene, the interspecific backcross was carried out by the mating (MRL/MpJ-lpr/lpr x MOL-TIT)x MRL/MpJ-lpr/lpr. Ly-44 (lymphocyte differentiation antigen-44) and Tdt (terminal deoxynucleotidyl transerase) were used as molecular marker genes on chromosome 19. RFLP of Ly-44 (lymphocyte differentiation antigen-44) and Tdt were in the case of BamHI. The following order of genes is proposed with the distances between genes given in parenthese: centromere - Ly-44 - (19.3cM) - lpr - (6.1cM) - Tdt - telomere. Therefore, the lpr gene is mapped at position 23 on mouse chromosome 19.