Genetic Ecology of Bradyrhizobium japonicum in soils

土壤中慢生根瘤菌的遗传生态学

• 批准号: 02455007
• 负责人: ASAMI Teruo
• 金额: $ 3.2万
• 依托单位: IBARAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02455007/
• 关键词: Bradyrhizobium; root nodule; soybean; host specificity; fingerprint; signal; genetic ecology; 根粒菌; DNA; フィンガ-プリント; 反復配列; 多様性

The isolates of Bradyrhizobium japonicum indigenous to six field in Japan were characterized by using four DNA hybridization probes from the species; nifDK, nodDYAB, hup, RS-alpha, and RS- beta. Diversity in RS-specific hybridization (RS-fingerprint) were observed. The RS-fingerprints were correlated with the nif and hup genotypes, and with the location of field including cropping history. According to nodDYAB hybridization, the field isolates were classified into three main group A, B and C. The group A and B produced similar nod metabolites including NodBj-V with methyl fucose. The group C isolates, which were separated only from siratro, were incompatible with soybeans,and produced different nod metabolites. As for nodulation, we constructed simple relationship. As a result, we established the method of genetic ecology for indigenous bradyrhizobia.

用4种日本慢生根瘤菌nifDK、node DYAB、HUP、RS-α和RS-β的DNA杂交探针对日本6个地区的慢生根瘤菌进行了鉴定。观察到RS特异杂交(RS指纹)的多样性。RS指纹图谱与NIF和HUP基因型相关，与包括种植历史在内的田间位置相关。根据Node DYAB杂交结果，将野生菌分为A、B、C三个主要组，A、B组产生相似的Nod代谢物，包括含有甲基岩藻糖的NodBj-V。仅从西里特罗分离的C组菌株与大豆不亲和，产生不同的NOD代谢物。对于结瘤，我们构建了简单的关系。因此，我们建立了本土慢生根瘤菌的遗传生态学方法。

项目成果

K.Minamisawa: "Phylogeny and ecology of Bradyrhizobium japonicum" Soil Microorganisms. 40. 37-46 (1992)

K.Minamisawa：“日本慢生根瘤菌的系统发育和生态学”土壤微生物。

Kiwamu Minamisawa: "Genetic structure of a indigenous Bradyrhizobium japonicum population as revealed by repeated sequences and various characteristics" Applied and Environmental Microbiology.

Kiwamu Minamisawa：“通过重复序列和各种特征揭示本土慢生根瘤菌种群的遗传结构”应用和环境微生物学。

南沢 究: "ダイズ根粒菌の系統と生態" 土の微生物. 40. 37-46 (1992)

Takashi Minamisawa：“大豆根瘤菌的系统学和生态学”土壤微生物。 40. 37-46 (1992)

南沢 究: "インド-ル酢酸生産能によるダイズ根粒菌の系統分類法" 日本土壌肥料学雑誌(第4号). 63. (1992)

Takashi Minamizawa：“基于吲哚乙酸生产能力的大豆根瘤菌的系统分类方法”《日本土壤与肥料杂志》（第 4 期）。（1992 年）。

Kiwamu Minanisawa: "Genetic Relatedness of Bradyrhizobium japonicum Field Isolates as Revealed by Repeated Saguencos and Various Other Characteristics" Applied and Environmental Microbiology. 58. 2832-2839 (1992)

Kiwamu Minanisawa：“重复萨金科斯和各种其他特征所揭示的日本慢生根瘤菌田间分离物的遗传相关性”应用和环境微生物学。