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Computer Control of Biodegradable Polymer Production Process by Alcaligenes eutrophus

真养产碱菌可生物降解聚合物生产过程的计算机控制

基本信息

批准号：
03453125
负责人：
SUGA Ken-ichi
金额：
$ 1.09万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

Kinetics of poly(-D(-)-3-hydroxyalkanoate) (PHA) production were studied in Alcaligenes eutrophus H16 (ATCC 17699) fed-batch culture using butyric acid and valeric acid as carbon souces. In poly(-D(-)-3-hydroxybutyric acid) (PHB) production, at the conditions of 3g/l butyric acid concentration and pH 8.0, the maximum values of specific production rate of PHB, production yield, and PHB content in the cell were obtained. Effect of cultivation condition on molecular weight distribution of PHB was studied. The average of molecular weight was decreased as the butyric acid concentration was increased. When the nitrogen source was added to the fermentation medium after adequate PHB accumulation in the cell, not only PHB production but also degradation were simultaneously occurred. The degradation rate of low molecular weight polymer was higher than that of high molecular weight polymer, and as a result, the average of molecular weight distribution shifted to the higher value. By addition of valeric acid to the butyric acid as carbon sources, copolyester of 3-hydroxybutyric acid (3HB) and 3-hydroxyvaleric acid (3HV), which is abbreviated as P(3HB-co-3HV), was produced but the specific production rate was lower than that of PHB.The mole fraction of P(3HB-co-3HV), was also investigated, using butyric acid and valeric acid as carbon sources. The mole fraction can be controlled within a range of compositions (0-40 mole% 3HV) by the mole ratio of butyric acid and valeric acid in the feeding mediums.

以丁酸和戊酸为碳源，研究了真养产碱杆菌（Alcaligenes eutrophus）H16（ATCC 17699）分批补料发酵生产聚D（-）-3-羟基烷酸酯（PHA）的动力学。在聚（-D（-）-3-羟基丁酸）（PHB）的生产中，丁酸浓度为3g/l，pH值为8.0时，比产率、产率和菌体中的含量均达到最大值。研究了培养条件对聚羟基丁酸酯分子量分布的影响。随着丁酸浓度的增加，平均分子量降低。当细胞中积累足够的PHB后，向发酵培养基中添加氮源时，不仅产生PHB，而且同时发生降解。低分子量聚合物的降解速率高于高分子量聚合物，因此，分子量分布的平均值向高值移动。以丁酸和戊酸为碳源，在一定条件下，丁酸和戊酸均能合成3-羟基丁酸（3 HB）和3-羟基戊酸（3 HV），但比产率低于聚羟基丁酸（PHB）。通过进料介质中丁酸和戊酸的摩尔比，可以将摩尔分数控制在组成范围（0-40摩尔% 3 HV）内。