Studies on regulatory factors for bacterial cell cycle.

细菌细胞周期调控因子的研究。

• 批准号: 03453137
• 负责人: NAGAI Kazuo
• 金额: $ 4.67万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03453137/
• 关键词: Escherichia coli; replication; cell division; oriC; F plasmid; SopA; mre; H-NS; oric; sopA; DNAー膜複合体; HーNS

Constituents of an oriC-complex of E. coli cells synchronized for initiation of chromosome replication were analyzed. 35-, 55-, 75-kDa proteins, found by Hendrickson et al. in the oriC-complex fractions, were revealed to be derived from glycogen particles and to be dispensable for formation of the complex (J.Bacteriol. 1992, 174: 5454-5456). As the candidates for real constituents of the complex, a histone-like protein H-Ns and a 17K component were newly detected in the oriC-complex fractions. A nucleate cell production by has mutant strain was observed microscopically. The 17K component seemed to be RNA.ATPase activity was detected in SopA protein, which is indispensable for stable maintenance F plasmid. The activity was enhanced by the addition of DNA and SopB protein (Mol. Gen. Genet. 1992, 234: 346-352).Changes in the amount of penicillin-binding proteins and other several proteins were observed in mreBCD mutant cells (J. Gen. Appl. Microbiol. 1992, 38: 157-163).

对E.分析了同步启动染色体复制的大肠杆菌细胞。Hendrickson等人在oriC-复合物级分中发现的35-、55-、75-kDa蛋白质被揭示为来源于糖原颗粒，并且被证实用于复合物的形成（J.Bacteriol. 1992，174：5454-5456）。作为复合物的真实的成分的候选者，在oriC复合物组分中新检测到组蛋白样蛋白H-Ns和17 K组分。显微镜下观察到突变株产生的有核细胞。SopA蛋白具有ATP酶活性，是维持F质粒稳定所必需的。通过添加DNA和SopB蛋白（Mol. Genet将军1992，234：346-352）。在mreBCD突变体细胞中观察到青霉素结合蛋白和其他几种蛋白的量的变化（J.Gen.Appl.Microbiol.1992，234：346-352）。1992，38：157-163）。

项目成果

和地 正明: "大腸菌細胞の伸長と分裂の制御機構" バイオサイエンスとインダストリ-. 49. 28-31 (1991)

Masaaki Wachi：“大肠杆菌细胞伸长和分裂的控制机制”《生物科学与工业》49. 28-31 (1991)。

永井 和夫,大森 斉: "細胞工学" 講談社, 186 (1992)

永井一夫、大森仁：《细胞工程》讲谈社，186（1992）

Akihiro Kaidow: "The 55-kilodalton protein in an oriC complex fraction is glycogen synthase" Journal of Bacteriology. 174. 5454-5456 (1992)

Akihiro Kaidow：“oriC 复合物组分中的 55 千道尔顿蛋白质是糖原合酶”《细菌学杂志》。

Michiko Ueki: "Escherichia coli mraR involved in cell growth and division" Journal of Bacteriology. 174. 7841-7843 (1992)

Michiko Ueki：“大肠杆菌 mraR 参与细胞生长和分裂”细菌学杂志。

Michiko Ueki: "Escherichia coli mraR gene involved in cell growth and division" Journal of Bacteriolgy. 174. 7841-7843 (1992)

Michiko Ueki：“大肠杆菌 mraR 基因参与细胞生长和分裂”细菌学杂志。