Studies on regulatory factors for bacterial cell cycle.

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Constituents of an oriC-complex of E. coli cells synchronized for initiation of chromosome replication were analyzed. 35-, 55-, 75-kDa proteins, found by Hendrickson et al. in the oriC-complex fractions, were revealed to be derived from glycogen particles and to be dispensable for formation of the complex (J.Bacteriol. 1992, 174: 5454-5456). As the candidates for real constituents of the complex, a histone-like protein H-Ns and a 17K component were newly detected in the oriC-complex fractions. A nucleate cell production by has mutant strain was observed microscopically. The 17K component seemed to be RNA.ATPase activity was detected in SopA protein, which is indispensable for stable maintenance F plasmid. The activity was enhanced by the addition of DNA and SopB protein (Mol. Gen. Genet. 1992, 234: 346-352).Changes in the amount of penicillin-binding proteins and other several proteins were observed in mreBCD mutant cells (J. Gen. Appl. Microbiol. 1992, 38: 157-163).