Pathogenesis of encephalomyocarditis virus-induced bi-phasic paralysis

脑心肌炎病毒所致双相性麻痹的发病机制

• 批准号: 03454105
• 负责人: DOI Kumio
• 金额: $ 3.52万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454105/
• 关键词: bi-phasic paralysis; encephalomyocarditis virus; mouse; helper; inducer T cell; macrophage; water-maze task performance; DNA probe; 脳心筋ウイルス; 後肢麻痺; 中枢神経病変

(1) Establishment of a model of bi-phasic paralysis We established a model of bi-phasic paralysis by inoculating 10^1PUF/head of EMC-D into 8-week-old male DBA/2 mice.(2) Sequence and pathogenesis of bi-phasic paralysis Transient hind limb paralysis developed by 10 days post inoculation (DPI) and it disappeared by 28 DPI. Thereafter recurrence of paralysis occured in about 30% of the recovered mice by 56 DPI. Virological, pathological and immunohistopathological examinations of the spinal cord revealed that direct viralattack to neurons and oligodendrocytes might play an important role in the development of initial paralysis and helper/inducer T cells and macrophages in the development of recurrent paralysis.(3) Water-maze task performance The influence of EMC-D on water-maze task performance was examined in DBA/2 and BALB/c mice. The disturbance of water-maze task performance observed in these mice might be partially due to the dysfunction of memory for working process or cognition of orientation other than underlying decreased ability in locomotion.(4) DNA probes for virus RNA of EMC-D DNA probes for virus RNA of WMC-D were made and usefulness of these DNA probes was confirmed.

(1)双相麻痹模型的建立我们用10^1PUF/头的EMC-D对8周龄雄性DBA/2小鼠建立双相麻痹模型。(2)双相麻痹的顺序和发病机制接种后10天（DPI）出现一过性后肢麻痹，28 DPI时消失。此后，到56 DPI时，约30%的恢复小鼠出现麻痹复发。脊髓的病毒学、病理学和免疫组织病理学检查表明，病毒对神经元和少突胶质细胞的直接攻击在最初麻痹的发生中起重要作用，辅助/诱导T细胞和巨噬细胞在复发性麻痹的发生中起重要作用。(3)在DBA/2和BALB/c小鼠中检测EMC-D对水迷宫任务表现的影响。这些小鼠的水迷宫任务表现障碍可能部分是由于工作过程记忆或方向认知功能障碍，而不是潜在的运动能力下降。(4)制备了EMC-D病毒RNA的DNA探针和WMC-D病毒RNA的DNA探针，并证实了这些DNA探针的有用性。

项目成果

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