Studies on prediction and inhibition for metastasis from carcinomas of the digestive tract

消化道癌转移的预测及抑制研究

• 批准号: 03454324
• 负责人: YAMADA Kazutaka
• 金额: $ 4.03万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454324/
• 关键词: Carcinoma of digestive tract; Invasive potentiality to the basement membrane; Invasion MTT assay; Type IV collagenase; Metastasis; Tumor xenografted in nude mice; 悪性度; 軽移; 浸潤; ヌ-ドマウス移植腫瘍

We have established the method of in vitro quantitative assay (invasion MTT assay) for studying invasive potentiality and metastatic behavior of the cancer cells using carcinomas of the digestive tract. The invasive potentiality to the basement membrane was estimated as percent invasion, that is the occurrence of the seeded cells in the cell chamber which penetrate through the basement membrane (percentage of the invaded cells to the seeded cells). The percent invasion values of tumors with liver metastasis were significantly higher than those of tumors without metastasis (p<0.001), although, it did not correlate with both type IV collagenase activity and cell capability of the adhesion, chemotaxis, and proliferation. The type IV collagenase activity was significantly higher in patients with liver metastasis, suggesting it has an important role to accelerate the liver metastasis.Clinicopathological parameters in 906 patients with carcinomas of the digestive tract were studied to know prediction capability for the liver metastasis using univariate analysis, resulting that vessel invasion and lymph node metastasis were the significant parameters. Using those parameters for the prediction, its specificity was more than 90% but sensitivity was less than 20%. Whereas, the percent invasion was measured using 30 tumors xenografted in nude mice obtained from the 906 patients. Its specificity for the prediction was 90% and sensitivity was 70%. An assessment of invasive potentiality to the basement membrane using the invasion MTT assay seemed to be useful method for predicting the liver metastasis form carcinomas of the digestive tract.

我们建立了体外定量检测方法(侵袭性四甲基偶氮唑盐比色法)，用于研究消化道癌细胞的侵袭潜能和转移行为。对基底膜的侵袭能力以侵袭百分率来估计，即在细胞室中出现穿透基底膜的种子细胞(侵袭细胞占种子细胞的百分比)。有肝转移的肿瘤的侵袭率显著高于无转移的肿瘤(p&lt；0.001)，但与IV型胶原酶活性及细胞的黏附、趋化和增殖能力无关。对906例消化道癌患者的临床病理参数进行单因素分析，发现血管侵袭和淋巴结转移是预测肝转移的重要指标。用这些参数预测，其特异度在90%以上，灵敏度在20%以下。然而，从906名患者身上获得的30例裸鼠移植瘤的侵袭率是测量的。其预测特异度为90%，敏感度为70%。用侵袭性的四甲基偶氮唑盐比色法评估基底膜的侵袭潜能是预测消化道癌肝转移的有用方法。

项目成果

Sonshin Takao: "Fumoignicity,invasion and metastasis of human gastric cancer in mde micl" Journal of Cancer Research Climcal Oncology. 117. 533-538 (1991)

Sonshin Takao：“mde micl 中人类胃癌的烟熏性、侵袭和转移”《癌症研究临床肿瘤学杂志》。

Shuichi Hokita: "Monoclonal antibodies against a human gastric cancer cell line with lung metastatic potential in nude mice define antigeus with defferent expression between the primary and metastatic liver lesions" Journal of Cancer Research Clinical Onc

Shuichi Hokita：“针对具有裸鼠肺转移潜力的人胃癌细胞系的单克隆抗体定义了原发性和转移性肝病灶之间具有不同表达的抗原”《癌症研究临床肿瘤杂志》

Shuichi Hokita: "monoclonal antibodies against a human gaotrci cancer cell line with lung metatrtci potential in nude nuce define antigaus with different expression betueen the primary and metastrtui liver las" Jourmal of Cancer Research Clinical Oncology

Shuichi Hokita：“针对裸露细胞中具有肺转移潜能的人高特奇癌细胞系的单克隆抗体定义了原发性和转移性肝脏之间具有不同表达的抗高蛋白”《癌症研究临床肿瘤学杂志》

Shuichi Hokita: "Monoclonal antibodies against a human gastric cancer cell line with lung metastatic potential in nude mice define antigens with different expression between the primary and metastatic liver lesions." Journal of Cancer Research Clinical On

Shuichi Hokita：“针对具有裸鼠肺转移潜力的人胃癌细胞系的单克隆抗体定义了原发性和转移性肝病灶之间表达不同的抗原。”

Shuichi Hokita: "Monoclonal Antibodies against a human gastric cancer cell live with long melaslatic potential in nude mice define antigeus with different expression between the primary and metastatic liven lesious" Journal of Cancer Research Chinical Onc

Shuichi Hokita：“抗人胃癌细胞的单克隆抗体在裸鼠体内具有长黄变潜能，定义了原发性和转移性肝病之间表达不同的抗原”《中国癌症研究杂志》