Hybrid-Massenspektrometer mit einem Gradienten Fokussierungssystem für die Flüssigkeitschromatographie

近年来，基于质谱学的蛋白质组学日益成为解决许多生物和医学问题的关键技术。该质谱仪具有灵敏度高、测序速度快、质量准确度高以及碰撞诱导解离(CID)、高能C陷阱解离(HCD)和电子转移解离(ETD)等多种碎裂技术。利用这种高分辨率的四极离子陷阱质谱仪，可以对复杂的生物样品进行深入的分析。除了配备高效液相色谱装置的标准配置外，还应用了另一种液相色谱系统，该系统是为高样品吞吐量和短梯度的项目而设计的。这个额外的层析单元将在科隆建立系统医学方面发挥重要作用。质谱仪还允许通过ETD对完整蛋白质进行裂解。到目前为止，这种方法在科隆大学是不可行的。不同类型的碎片(CID，ETD)的组合也提供了通过质谱学可裂解的交联剂分子来表示交联肽的最佳表示。在与生物化学系的密切合作下，将结合使用交联质谱(XL-MS)和低温电子显微镜来研究蛋白质复合体的结构。CID碎裂将使用离子陷阱质谱仪(LTQ-Orbitrap Discovery；购买于2008年)，这已不再是最先进的技术。总体而言，拟议的质谱计将增加该设施的容量，并显著扩大质谱学方法的范围。

In recent years, mass spectrometry-based proteomics has increasingly become the key technology for many biological and medical questions. The applied mass spectrometer possesses high sensitivity, fast sequencing speed, high mass accuracy and diverse fragmentation techniques such as collision-induced dissociation (CID), higher-energy C-trap dissociation (HCD) and electron-transfer dissociation (ETD). Using this high-resolution quadrupole ion-trap mass spectrometer, complex biological samples can be analyzed in depth. In addition to the standard configuration with a high-performance liquid chromatography device, a further liquid chromatography system was applied for, which was designed for projects with high sample throughput and short gradients. This additional chromatography unit will play an important role in the establishment of systems medicine in Cologne. The mass spectrometer also allows the fragmentation of intact proteins via ETD. So far, this method is not feasible at the University of Cologne. The combination of different types of fragmentation (CID, ETD) also offers an optimal representation of cross-linked peptides by mass spectrometry-cleavable cross-linker molecules. In close cooperation with the biochemistry department, the structure of protein complexes will be investigated using a combination of cross-linking mass spectrometry (XL-MS) and cryo-electron microscopy. CID fragmentation will be performed with an ion trap mass spectrometer (LTQ-Orbitrap Discovery; purchased 2008), which is no longer state of the art. Overall, the proposed mass spectrometer will increase the capacity of the facility and significantly expand the range of mass spectrometric methods.