Hybrid-Massenspektrometer mit einem Gradienten Fokussierungssystem für die Flüssigkeitschromatographie

具有液相色谱梯度聚焦系统的混合质谱仪

• 批准号: 431335494
• 金额: --
• 依托单位: Universität zu Köln
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/431335494?language=en
• 关键词: Hybrid; Massenspektrometer; mit; einem; Gradienten

In recent years, mass spectrometry-based proteomics has increasingly become the key technology for many biological and medical questions. The applied mass spectrometer possesses high sensitivity, fast sequencing speed, high mass accuracy and diverse fragmentation techniques such as collision-induced dissociation (CID), higher-energy C-trap dissociation (HCD) and electron-transfer dissociation (ETD). Using this high-resolution quadrupole ion-trap mass spectrometer, complex biological samples can be analyzed in depth. In addition to the standard configuration with a high-performance liquid chromatography device, a further liquid chromatography system was applied for, which was designed for projects with high sample throughput and short gradients. This additional chromatography unit will play an important role in the establishment of systems medicine in Cologne. The mass spectrometer also allows the fragmentation of intact proteins via ETD. So far, this method is not feasible at the University of Cologne. The combination of different types of fragmentation (CID, ETD) also offers an optimal representation of cross-linked peptides by mass spectrometry-cleavable cross-linker molecules. In close cooperation with the biochemistry department, the structure of protein complexes will be investigated using a combination of cross-linking mass spectrometry (XL-MS) and cryo-electron microscopy. CID fragmentation will be performed with an ion trap mass spectrometer (LTQ-Orbitrap Discovery; purchased 2008), which is no longer state of the art. Overall, the proposed mass spectrometer will increase the capacity of the facility and significantly expand the range of mass spectrometric methods.

近年来，基于质谱的蛋白质组学技术日益成为解决许多生物学和医学问题的关键技术。所应用的质谱仪具有灵敏度高、测序速度快、质量准确度高和多种裂解技术，如碰撞诱导解离（CID）、高能C阱解离（HCD）和电子转移解离（ETD）。使用这种高分辨率四极杆离子阱质谱仪，可以对复杂的生物样品进行深入分析。除了配备高效液相色谱装置的标准配置外，还应用了另一种液相色谱系统，该系统专为具有高样品通量和短梯度的项目而设计。这一新增的色谱单元将在科隆系统医学的建立中发挥重要作用。质谱仪还允许通过ETD裂解完整蛋白质。到目前为止，这种方法在科隆大学还不可行。不同类型的片段化（CID、ETD）的组合还通过质谱可裂解的交联剂分子提供交联肽的最佳表示。与生物化学系密切合作，将使用交联质谱（XL-MS）和冷冻电子显微镜的组合研究蛋白质复合物的结构。CID裂解将使用离子阱质谱仪（LTQ-Orbitrap Discovery; 2008年购买）进行，该质谱仪不再是最先进的。总体而言，拟议的质谱仪将增加设施的容量，并显著扩大质谱方法的范围。