Medico-Legal Study on the Systematic and Microscopic Procedure for Wound Examination

伤口检查在法医病理学中是最重要的，法医病理学家经常被要求评估伤口是如何造成的，使用了哪种类型的武器。此外，与死亡时间一样，客观地确定伤口是死前还是死后，以及如果是死前，在死亡前持续了多长时间，一直是尸检的主要问题。本研究的目的是建立一种显微的、系统的伤口检查程序，以便发现和记录各种创伤，并更准确、客观地估计皮肤伤口的年龄。1.显微伤口检查程序的法医学法律研究在法医尸检案件中，使用双目手术显微镜对人体皮肤伤口进行显微观察。因此，使用手术显微镜的伤口检查在确定擦伤的撞击方向、区分撕裂伤和切割伤…方面特别有用2.皮肤损伤后创面年龄估计的实验研究本研究通过与I型胶原、纤维连接蛋白等细胞外基质的比较，探讨IL-1α的免疫组织化学定位，并探讨IL-1α随时间推移的定位分析是否可以为创面年龄的判断提供进一步的信息。因此，在创伤后3小时至1天之间，即创面愈合的急性炎症阶段，用免疫组织化学方法确定IL-1α的定位似乎是非常有用的。此外，I型胶原和纤维连接蛋白在伤口愈合的增殖期和成熟期也是有用的标志物。通过免疫组织化学染色IL-1α以及I型胶原和纤维连接蛋白，似乎有可能更准确和客观地估计皮肤创伤的年龄。较少

Wound examination is of prime importance in forensic pathology, and forensic pathologists are often required to assess how a wound was made and with which type of weapon. Furthermore, like the time of death, it is always main problem at autopsy to determine objectively whether a wound was ante-mortem or post-mortem, and if ante-mortem, how long before death it had been sustained. The purpose of this study is establishment of a microscopic and systematic procedure for wound examination in order to detect and record wounds of various kinds, and also to estimate skin wound age more accurately and objectively.1.Medico-legal study on the microscopic wound examination procedureHuman skin wounds were microscopically observed by the use of a binocular operation microscope in forensic autopsy cases. In result, wound examination using the operation microscope is particularly useful in the determination of the direction of impact in abrasions, differentiation between lacerations and incised wound … More s and between the two ends of a stab wound damaged by the blade and the blunt side of a single-edge knife, the close observation of old scars and blood vessel lesions, and the training of forensic pathologists and clinical physicians.2.Experimental study on the estimation of the skin wound age after injuryThe study was performed to elucidate immunohistochemical localization of IL-1alpha through the comparison with that of extracellular matrix such as collagen type I and fibronectin, and to discuss whether tha analysis of IL-1alpha localization with the passage of time may provide further information for the wound age estimation. In result, it seems to be much useful to immunohistochemically identify interleukin 1alpha localization when the determination of wound age between 3 hours and 1 day after injury, that is, in acute inflammatory phase of wound healing is needed. And, collagen type I and fibronectin are also useful as markers in the proliferative and maturation phase of wound healing. It seems possible, by immunostaining IL-1alpha as well as collagen type I and fibronectin, to estimate skin wound age more correctly and objectively. Less