权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

DEVELOPMENT OF EARLY DETECTION OF ADULT RESPIRATORY DISTRESS SYNDROME

成人呼吸窘迫综合征早期检测的进展

基本信息

批准号：
04454253
负责人：
SUZUKI Shunsuke
金额：
$ 3.71万
依托单位：
YOKOHAMA CITY UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

In adult respiratory distress syndrome (ARDS), the decrease in activity of surfaceactive material (surfactant), which increases the alveolar surface tension and decrease the alveolar surface-to-volume (S/V) ratio, is observed in the early stage. In our previous study, we developed the light scattering stereology which measures the optical mean free path, lambda(equivalent to an inverse of the S/V ratio). In the present study, we aimed to develop a detection method of permeability pulmonary edema in ARDS,using the light-scattering stereology.In mechanically ventilated dogs with open chest, we modified the light-scattering stereology, and were able to measure lambda continuously. The S/V ratio was smaller in inspiration than in expiration, suggesting a greater surface tension in inspiration. In ventilated lungs, the surface tension became less than the tissue force of the septa as the ventilatory frequency increases.To examine the effect of surfactant on the S/V ratio, we washed the lung with saline. After saline lavage, the S/V ratio decreased, suggesting that the alveolar surface tension increases by lavage of surfactant. In ARDS model of oleic acid (OA) -induced pulmonary edema, the S/V ratio decreased but the saline-filled pressure-volume curve did not differ between control and OA-induced edema. It is likely that the activity of surfactant may decrease by exudation of plasma, resulting in an increase in alveolar surface tension. In dogs injected by lipopolysaccharide (LPS), the S/V ratio decreased before hypoxemia and the decrease in lung compliance occurred. Thus, light-scattering stereology is able to measure the S/V in live animals and detect the change in surface tension. We concluded that pulmonany edema may be detected in early phase by using the light-scattering stereology.

在成人呼吸窘迫综合征（ARDS）中，早期观察到表面活性物质（表面活性剂）活性降低，从而增加肺泡表面张力并降低肺泡表面与体积（S/V）比。在我们之前的研究中，我们开发了光散射体视学，用于测量光学平均自由程 lambda（相当于 S/V 比的倒数）。在本研究中，我们旨在开发一种利用光散射体视学检测ARDS中渗透性肺水肿的方法。在机械通气的开胸犬中，我们修改了光散射体视学，并且能够连续测量λ。吸气时的 S/V 比小于呼气时的 S/V 比，表明吸气时的表面张力更大。在通气的肺中，随着通气频率的增加，表面张力变得小于隔膜的组织力。为了检查表面活性剂对S/V比的影响，我们用盐水清洗肺。盐水灌洗后，S/V 比值下降，表明表面活性剂灌洗导致肺泡表面张力增加。在油酸 (OA) 诱导的肺水肿的 ARDS 模型中，S/V 比下降，但对照组和 OA 诱导的水肿之间的盐水填充压力-容量曲线没有差异。表面活性剂的活性可能会因血浆渗出而降低，导致肺泡表面张力增加。在注射脂多糖（LPS）的狗中，在发生低氧血症和肺顺应性降低之前，S/V比值降低。因此，光散射体视学能够测量活体动物的 S/V 并检测表面张力的变化。我们的结论是，通过使用光散射体视学可以在早期检测到肺水肿。