Molecularbiological studies of the mechanism on the abnormal prostatic growth : Epithelial-mesenchymal interaction

(1)胎儿尿生殖窦间充质通过上皮-间充质相互作用诱导所有前列腺分泌蛋白产物以及任何成年啮齿动物前列腺叶上的前列腺上皮生长。采用无血清条件培养液培养小鼠前列腺器官，观察角质细胞生长因子（KGF）对前列腺发育的影响。这一结果表明，KGF作为上皮细胞的旁分泌介质的作用，它是由间充质细胞产生的。(2)新生代精囊间充质引起Dunning大鼠前列腺癌生长速度减慢、上皮形态、分泌蛋白分子量和睾酮代谢模式的改变。这种类似前列腺或精囊腺的诱导特征与正常前列腺或精囊腺相似。这些发现表明，结缔组织环境可以对正常和肿瘤上皮细胞具有深刻的调节作用。像KGF这样的旁分泌介质肯定会增加我们对致癌过程的了解，并导致新的治疗策略。

(1) The fetal urogenital sinus mesenchyme induced all prostatic secretory proteins product as well as the prostatic epithelial growth on any adult rodent prostatic lobes via epithelial-mesenchymal interaction. We examined the role of Keratinocyte growth factor (KGF) for prostatic development with mouse prostatic organ culture system using the serum free conditioning medium. That results suggested the role of KGF as a paracrine mediator for epithelial cells, which was produced by mesenchymal cells.(2) Neonatal seminal vesicle mesenchyme elicited the reduction of growth rate and the changes of epithelial morphology, secretory protein molecular weight and testosterone metabolic pattern on Dunning rat prostatic carcinoma. Such like induced characteristics were similar to those of normal prostate or seminal vesicle. These findings demonstrate that the connective tissue environment can have profound regulative effects on both normal and neoplastic epithelial cells. The paracrine mediators like a KGF might certainly augment our knowledge of the carcinogenic process and lead to new therapeutic strategies.