Molecularbiological studies of the mechanism on the abnormal prostatic growth : Epithelial-mesenchymal interaction

前列腺异常生长机制的分子生物学研究：上皮-间质相互作用

• 批准号: 04454401
• 负责人: KAWAMURA Juichi
• 金额: $ 4.67万
• 依托单位: Mie university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454401/
• 关键词: Epithelial-mesenchymal interaction; Recombination; Urogenital sinus mesenchyme; Cell growth; Secretory protein; Dunning rat tumor; Organ culture; Keratinocyte Growth Factor; 上皮-間質相互作用; 合成エストロゲン; 異常前立腺腺管; テストステロン代謝

(1) The fetal urogenital sinus mesenchyme induced all prostatic secretory proteins product as well as the prostatic epithelial growth on any adult rodent prostatic lobes via epithelial-mesenchymal interaction. We examined the role of Keratinocyte growth factor (KGF) for prostatic development with mouse prostatic organ culture system using the serum free conditioning medium. That results suggested the role of KGF as a paracrine mediator for epithelial cells, which was produced by mesenchymal cells.(2) Neonatal seminal vesicle mesenchyme elicited the reduction of growth rate and the changes of epithelial morphology, secretory protein molecular weight and testosterone metabolic pattern on Dunning rat prostatic carcinoma. Such like induced characteristics were similar to those of normal prostate or seminal vesicle. These findings demonstrate that the connective tissue environment can have profound regulative effects on both normal and neoplastic epithelial cells. The paracrine mediators like a KGF might certainly augment our knowledge of the carcinogenic process and lead to new therapeutic strategies.

(1)胎儿尿生殖窦间充质通过上皮-间充质相互作用诱导所有前列腺分泌蛋白产物以及任何成年啮齿动物前列腺叶上的前列腺上皮生长。采用无血清条件培养液培养小鼠前列腺器官，观察角质细胞生长因子（KGF）对前列腺发育的影响。这一结果表明，KGF作为上皮细胞的旁分泌介质的作用，它是由间充质细胞产生的。(2)新生代精囊间充质引起Dunning大鼠前列腺癌生长速度减慢、上皮形态、分泌蛋白分子量和睾酮代谢模式的改变。这种类似前列腺或精囊腺的诱导特征与正常前列腺或精囊腺相似。这些发现表明，结缔组织环境可以对正常和肿瘤上皮细胞具有深刻的调节作用。像KGF这样的旁分泌介质肯定会增加我们对致癌过程的了解，并导致新的治疗策略。

项目成果

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Sugimura Y 等人：“衰老加速小鼠中前列腺的年龄相关变化”前列腺。

Hayashi N,et al.: "Permissive and instructive induction of adult rodent prostatic epithelium by heterotypic urogenital sinus mesenchyme." Epith Cell Biol.2. 66-78 (1993)

Hayashi N 等人：“异型泌尿生殖窦间充质对成年啮齿动物前列腺上皮的许可性和指导性诱导。”

Yamashita A,et al.: "Influence of beta-diethylstilbestrol, Leuprolelin (an LH-RH analogue), Finasteride (a 5alpha-reductase inhibitor) and castration on the lobar subdivisions of the rat prostate." Prostate accepted. (1995)

Yamashita A 等人：“β-己烯雌酚、亮丙瑞林（LH-RH 类似物）、非那雄胺（5α-还原酶抑制剂）和去势对大鼠前列腺叶区的影响。”

Kinbara H,et al.: "Ductal heterogeneity in rat dorsal-lateral prostate" Prostate submitted. (1995)

Kinbara H 等人：“大鼠背外侧前列腺的导管异质性”前列腺提交。

YC.Wong,GR Cunha N.Hayashi: "Effects of mesenchyme of the embryonic urogenital sinus and neonatal seminal vesicle one the cytodifferentiation of the Duning tumor:Ultrastructural study" Acta Anatomica. 143. 139-150 (1992)

YC.Wong，GR Cunha N.Hayashi：“胚胎泌尿生殖窦和新生儿精囊间充质对杜宁肿瘤细胞分化的影响：超微结构研究”《解剖学报》。