The study to the role of extra-cellular marorix in the metastasis and invesion of malignat tumor of gynecological field

背景资料。比较子宫内膜癌、卵巢癌和正常组织中导致细胞外基质降解的蛋白水解酶活性。肿瘤细胞和正常细胞的条件培养液在以明胶为底物的十二烷基硫酸钠-聚丙烯酰胺凝胶上进行电泳。凝胶电泳后，考马斯亮蓝染色，用密度法分析酶的活性，以染料透明带表示。Northern印迹分析检测TIMPmRNA的表达。密度计量学分析表明，所有的子宫内膜癌都表达了很高的分子量酶活性(Mr 220,000)，而在正常子宫内膜细胞的培养液中没有检测到这一活性。分析还表明，在子宫内膜癌中，mR 92,000酶的活性总是高于mR 64,000酶的活性，这与正常子宫内膜的情况相反。在卵巢癌中，92 KDa明胶酶/IV型胶原酶的活性与正常卵巢的情况相反。Northern印迹分析显示，TIMPmRNA在卵巢癌组织和正常卵巢组织中的表达无明显差异。这些结果表明，MR 220,000酶活性的表达和MR 92,000酶活性高于MR 64,000酶的活性与子宫内膜癌的恶性表型有关。在卵巢癌中，92 KDa明胶酶/IV型胶原酶活性高于64 KDa酶，参与了卵巢癌的恶性表型；而这些酶的抑制剂TIMP在组织中广泛分布，其水平与卵巢癌的恶性程度无关。

Background. The protease activity leading to degradation of the extracellular matrix was compared between human endometrial and ovarien cancer and normal tissue.Methods. Conditioned medium from tumor cells and nomal cells was subjected to electrophoresis on sodium dodecyl sulfate(SDS)-polyacrylamide gel containing gelatin as a substrate. After electrophoresis, the gel was stained with Coomassie blue, and then the enzume activity, expressed as the zone of dye clearing, was analyzed by densitomery. The expression of TIMPmRNA was examined by Northern blot analysis.Results. Densitometric analysis showed that all the endometrial cancers expressed a very high molecular weight enzume activity (Mr 220,000), which was not detected in medium from normal endometrial cells. The analysis also showed that in endometrial cancer the activity of a Mr 92,000 enzyme was always superior to that of a Mr 64,000 enzyme, which was in contrast to the situation for normal encometrium. In ovarian cancer, the activity of a 92KDa gelatinase/type IV collagenase in contrast to the situation in the noomal ovary. Northern blot analysis revealed no remarkable difference of TIMPmRNA expression between cancer and normal ovarian tissues.Conclusions. These results indicate that the expression of Mr 220,000 enzume activity and the higher activity of the Mr92,000 enzume than the Mr64,000 enzyme are involved in the malignant phenotype of native endometrial cancer. In ovarian cancer, the higher activity of the 92KDa gelatinase / typeIV collagenase enzume, relative to that of the 64KDa enzyme, is involved in the malignant phenotype of ovarian cancer ; while the inhibitor of these enzymes, TIMP, is distributed in a widespread fashion in the tissue, and its levels are not correlated with the malignancy.