THE STUDY OF ETIOLOGY AND PATHOPHYSIOLOGY OF PREECLAMPSIA,ANALYSIS IN THE ASPECT OF INTRACELLULAR SIGNAL TRANSDUCTION

1.内皮细胞功能：重度妊高征患者血浆内皮素-1水平明显高于正常对照组。在重度妊高征中也观察到脂肪代谢的变化如下：高脂血症伴低HDL-胆固醇血症、血浆饱和/不饱和脂肪酸比增加、血浆脂质过氧化物浓度增加和血浆维生素E水平降低。这些被认为是引发和/或恶化内皮细胞功能障碍的因素。使用培养的内皮细胞进行的体外研究也表明，重度妊高征患者性类固醇和脂质的变化与该疾病的病理生理学有关。然而，重度妊高征患者也表现出血小板活化因子的血浆浓度增加，其具有血管舒张作用，这表明内皮细胞可能仍然具有拮抗高血压的功能。一氧化氮合酶 关于我们 Western blot结果显示，正常妊娠组与重度妊高征组胎盘组织组成型基本相同，重度妊高征组尿一氧化氮代谢产物排出量虽较少，但有减少的趋势.电解质代谢：重度妊高征患者全血离子镁和细胞内镁浓度均显著低于正常对照组，而血清镁浓度两组间无显著性差异。提示重度妊高征患者存在镁代谢的改变，MgSO 4负荷试验结果显示重度妊高征患者存在镁的相对缺乏.胎儿发育：血浆IGF-1水平沿着正常妊娠过程增加，与胎儿发育和母体体重增加相关。在严重妊高征的IGF-1血浆浓度是非常低的，尽管母亲体重增加。这表明IGF-1的变化反映了母亲营养状况的紊乱，这与胎儿生长密切相关。少

1. Endothelial cell function : Pregnant women complicated with pregnancy-induced hypertension in severe type (severe PIH) showed increased plasma levels of endothelin-1 than did normal controls. Changes in fat metabolism were also observed in severe PIH as follows ; higher triglyceridemia with lower HDL-cholesterolemia, increased plasma saturated/unsaturated fatty acids ratio, increased plasma concentration of lipid-peroxide and decreased levels of plasma vitamin E.These are considered as factors intiating and/or deteriorating endothelial cell dysfunction. In vitro studies using cultured endothelial cells also demonstrated that altered profiles of sex steroids and that of lipids in the severe PIH are involved in the pathophysiology of the disorder. However, severe PIH patients also showed increased plasma concentration of platelet-activating factor, which has vasodilatory effect, suggesting that endothelium may still function to antagonize high blood pressure. Nitric oxide synthase of … More placental tissue in constitutive type was demonstrated to be equal on Western blot between normal pregnancy and severe PIH.Although the number of cases is small, there was a tendency of decreased urinary excretion of metabolites of nitric oxide in severe PIH.2. Electrolytes metabolism : Concentrations of ionized Mgin whole blood and these of intracellular Mg were significantly lower in severe PIH than in normal controls, while there was no significant difference in serum Mg levels between the two groups. These suggest altered Mg metabolism exist in severe PIH.The results of MgSO4 loading revealed a relative deficiency in Mg in the disorder.3. Fetal development : Plasma IGF-1 levels increased along the course of normal pregnancy correlating to fetal development and maternal weight gain. In severe PIH plasma concentrations of IGF-1 was extremely low in spite of maternal weight gain. This suggests change in IGF-1 reflects disorders in maternal circumstances in nutrition which is closely related to fetal growth. Less