Studies on the mechanism of autoimmune poliglandular endocrine disorders determined ny antipituitary antibodies

ny抗垂体抗体测定自身免疫性多腺体内分泌紊乱的机制研究

• 批准号: 04454545
• 负责人: KOBAYASHI Isao
• 金额: $ 4.03万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454545/
• 关键词: antipituitary antibodies.; pituitary dwarfism.; isolated ACTH deficiency.; pituitary antibodies.; Graves' disease. (6) Hashimoto's thyroiditis.; 橋本病; 自己免疫性疾患

We have established an assay method by western Blot analysis of antipituitary antibodies. and evaluated on clinical significance of determination of antipituitary antibodies.Antigens was obtained from rat pituitary cytosolic fractions. 50 ul of sample(200 ug protein) was mixed with 50 ul of smple buffer. Searated proteins were transfered to a PVDF membrane by a semi-dry blotting apparatus. The membrane was incubated in 1 : 101 diluted human serum.We have fund 22 kDa positive band after identification of pituitary antigens recognized by antipituitary antibodies. The prevalence of positive oituitary antibodies by this method was 40.0%(12/30) in patents with insulin-dependent diabetes melltus, while nobody showed positive results in patients withnon-insulin dependent diabetes mellitus. The 22 kDa positive band was found in 14 of 53 patients with Graves' disease(26.4%) and 12 of 54 patients with Hashimoto's thyroditis(22.2%). Eleven of 25 patients with pituitary dwarfism showed positive antibodies(44.0%).The 22 kDa protein was considered to be GH-related protein in rat pituitary cytosolic fractions.Thus, we have demonstrated a new assay system by western Blot analysis of antipituitary antibodies. We are now in progress to determine pituitary cell surface membrane antibodies.

我们建立了一种抗垂体抗体的免疫印迹分析方法。从大鼠垂体胞浆中提取抗垂体抗体，并对抗垂体抗体测定的临床意义进行评价。将50 μ l样品（200 μ g蛋白质）与50 μ l单一缓冲液混合。用半干印迹仪将蛋白质转移到PVDF膜上。将膜在1：101稀释的人血清中孵育，经抗垂体抗体鉴定，发现22 kDa的阳性条带。胰岛素依赖型糖尿病患者血清抗体阳性率为40.0%（12/30），非胰岛素依赖型糖尿病患者血清抗体阴性。53例Graves病和54例桥本甲状腺炎患者中，22 kDa阳性条带分别为14例（26.4%）和12例（22.2%）。25例垂体性侏儒症患者中11例（44.0%）抗垂体抗体阳性，该22 kDa蛋白被认为是垂体细胞浆中的GH相关蛋白。我们现在正在进行垂体细胞表面膜抗体的测定。

项目成果

小林功,他: "下垂体系:特に下垂体抗体をめぐって" 臨床病理. 41. 123-127 (1993)

Isao Kobayashi 等：“垂体系统：特别是关于垂体抗体”《临床病理学》41. 123-127 (1993)。

小林功,他: "下垂体系:特に下垂体抗体をめぐって" 臨症病理. 41. 123-127 (1993)

Isao Kobayashi 等：“垂体系统：特别是关于垂体抗体”《临床病理学》41. 123-127 (1993)。

Isao Kobayashi, et al.: "Pituitary system : With special reference to pituitary antibodies" Jaanese Journal of Clinical Pathology.Vol.41. 123-127 (1993)

Isao Kobayashi 等人：“垂体系统：特别提及垂体抗体”Jaanese Journal of Clinical Pathology.Vol.41。

Isao Kobayashi, et al.: "Assay method of antipituitary antibodies" Nihon Rinsho. Vol.51. 2673-2678 (1993)

Isao Kobayashi 等：“抗垂体抗体的测定方法”Nihon Rinsho。

小林他: "抗下垂体抗体の測定法" 日本臨床. 51. 2673-2678 (1993)

小林等人：“抗垂体抗体的测定方法”日本临床，51.2673-2678(1993)。